Description

RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining reagent, a substitute for EtBr (ethidium bromide)
The RedSafe™ Nucleic Acid Staining Solution exhibits two fluorescence excitation maxima at 309 nm and 419 nm when bound to nucleic acids and is visible at a wavelength of 514 nm. RedSafe™ Nucleic Acid Staining Solution is as sensitive as EtBr and its usage is similar to EtBr. Compared with EtBr, a potent carcinogen, RedSafe™ Nucleic Acid Staining Solution showed almost no mutation in the Ames test and negative results in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. Therefore, we strongly recommend that you select RedSafe™ Nucleic Acid Stainig Solution (20,000x) instead of EtBr to identify nucleic acids on agarose gel.

Applications

• 01 Separated by agarose gel electrophoresis Identification of DNA / RNA bands
• 02 Without mutation caused by EtBr Separate the subcloned DNA fragment

Storage

• 01RT (Room Temperature)





 

Experimental Informations




※ Spectrum

1. Excitation wavelength (EX)

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Fig. 1. Measurement of Fluorescence Excitation Wavelength

RedSafe™ Nucleic Acid Staining Solution (20,000x) has two fluorescence excitation maxima, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm.





  2. Emission wavelength (EM)

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Fig. 2. Measurement of fluorescence emissionwavelength

The fluorescence emission of RedSafe™ Nucleic Acid Staining Solution (20,000x) bound to DNA is centered at 537 nm.

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