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Real-time PCR

Life Science (Reagent) Amplification Reagent Real-time PCR

RealMOD™ Probe M² 2X qPCR mix

Cat.No Capacity Inquire
25359.100 100 rxn Inquire
25359.500 500 rxn Inquire
25359.1000 1000 rxn Inquire
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PRODUCT INFORMATION
Description
It is composed of TaqMan Probe type, and it is a real-time PCR (qPCR) that can be easily performed using DNA as a template.
• Real-time PCR reaction volume can be controlled with 2X Master mix type
• Hot-start function is included to minimize non-specific reactions
• Excellent reproducibility and stability
• Excellent qPCR results even in templates with high GC contents
RealMOD™ Probe M² 2X qPCR mix is a TaqMan Probe type product that can perform quantitative PCR using cDNA or gDNA.
This product has a hot-start function, so the dimer generation rate is low and the reproducibility and stability are high. Also in templates with high GC contents
The qPCR result is excellent, and it is provided as a 2X Master mix type, so the dose can be adjusted according to the experimenter.​​
Applications
  • 01Detection and quantification of DNA and cDNA target
  • 02Gene-expression analysis
  • 03Pathogene detection and quantification
Kit Contents
ContentVolumes
RealMOD™ Probe ​M²​ 2X qRT-PCR mix1 ml
Manual1 ea
Wide Instruments Compatibility
The RealMOD™ Probe M² 2x qPCR mix is standard qPCR capable. The types of devices that can be tested using this product are as follows.

• Applied BioSystems: QuantStudio™ 12K Flex, ViiA™ 7, 7900HT, 7500, 7700, StepOne™ & StepOne plus™
• Stratagene : MX3000P™, MX3005P™
• Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpicon®
• Qiagen : Rotor-Gene® Q, Rotor-Gene® 6000
• Eppendorf : Mastercycler® ep realplex2 & ep realplex4
• Roche: LightCycler®480
• Illumina: The Eco™​

     

Technical Data

 

  

Figure 1. Amplification of ASFV plasmid DNA using RealMOD™ Probe M² 2x qPCR mix

ASFV plasmid DNA was serial diluted 1/10 (Top, Top-1, Top-2, Top-3, Top-4 , Top-5).

Amplification of plasmid DNA using RealMOD™ Probe M² 2X qPCR mix on an CFX-96 Real-time PCR system.​ 

 

Figure 2. Amplification of salmonella plasmid DNA using RealMOD™ Probe M² 2x qPCR mix

Salmonella plasmid DNA was serial diluted 1/10 (Top, Top-1, Top-2, Top-3, Top-4 , Top-5).

Amplification of plasmid DNA using RealMOD™ Probe M² 2X qPCR mix on an CFX-96 Real-time PCR system.​

 

 


Figure 3. Amplification of listeria plasmid DNA using RealMOD™ Probe M² 2x qPCR mix

Listeria plasmid DNA was serial diluted 1/10 (Top, Top-1, Top-2, Top-3, Top-4 , Top-5).

Amplification of plasmid DNA using RealMOD™ Probe M² 2X qPCR mix on an CFX-96 Real-time PCR system.

 

 

 

- As a result of the real-time PCR experiment, a dynamic range suitable for the dilution factor (1/10) was observed.

- It has been confirmed that the reliability of the standard curve is very high and the PCR efficiency is also excellent.​ 


 

TroubleShooting Guide

 

QAs a result of the experiment, amplification cannot be confirmed.
AFirst, please check whether there is any problem in the template extraction process. Second, please check if there are any missing contents (primer, probe, 2X qRT-PCR mix) when preparing the PCR mixture. Third, check the experimental conditions by reviewing the primer design and the annealing temperature of the prime.​

 


QAs a result of the experiment, a signal was detected in the NC (negative control).
AFirst, non-specific reactions may occur due to environmental factors, so please cleanly disinfect the equipment and test table being used before proceeding. Second, non-specific reactions may occur due to experimental factors (contamination by the template to be used), so please make a mixture, dispense negative control first, and then conduct an experiment on the target.​

 


QIs ROX dye added to the product?
AROX dye is not added to this product, and if necessary, it should be added after use.​
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