Diagnostic Specificity

100% Target Specific

Subtyping Coverage

HA 1-16 & NA 1-9

Fast Turnaround

Within 90 - 120 Mins

Platform Synergy

Real-time + Multi-tube

1

Understanding AIV Diagnostic Targets

UNIVERSAL SCREENING

NP · M Gene

Conserved Internal Nucleoprotein & Matrix

Represents highly stable internal genomic regions of Influenza A virus. Essential as the first-line screening marker for reliable detection of all avian influenza strains regardless of mutation status.

SURFACE SUBTYPING

HA 1-16 Subtypes

Hemagglutinin Glycoprotein Antigens

Mediates host cell receptor binding and viral entry. Divided into 16 distinct avian HA subtypes (H1-H16). Focuses particularly on high-risk zoonotic and poultry-lethal strains such as H5, H7, and H9.

SURFACE SUBTYPING

NA 1-9 Subtypes

Neuraminidase Glycoprotein Enzymes

Catalyzes cleavage of sialic acid to enable progeny virus budding and transmission. Differentiates N1 through N9 subtypes for complete phylogenetic classification (e.g., H5N1, H5N6, H7N9).

2

LiliF™ AIV Product Lineup at a Glance

TAQMAN PROBE TECHNOLOGY

Real-time RT-PCR Series

High-sensitivity M gene universal screening and selective H5 / H7 differentiation. CE-IVD and medical device approved for clinical & quarantine accuracy.

MAXIME™ DRY PREMIX TECHNOLOGY

Multi-tube RT-PCR Series

Multiplex AIV screening and comprehensive HA 1-16 / NA 1-9 subtyping. Designed for epidemiological strain investigation and national livestock defense.

3

Strategic Selection & Application Guide

WHEN TO CHOOSE REAL-TIME RT-PCR

Routine Surveillance & Rapid Emergency Screening

- Target Audience: Veterinary reference labs, poultry breeding facilities, international quarantine stations requiring rapid turnaround.
- Key Strength: High sensitivity probe detection, automated closed-tube workflow with zero post-PCR contamination risk, rapid time-to-result (~90 mins).
- Recommended Kits: AIV M Kit (IP15004) for universal gatekeeping, followed immediately by H5 Kit (IP15005) or H7 Kit (IP15006) for HPAI risk verification.

WHEN TO CHOOSE MULTI-TUBE RT-PCR

Comprehensive Subtyping & Outbreak Investigation

- Target Audience: National animal health institutes, epidemiological researchers, and labs managing diverse or unknown avian viral strains.
- Key Strength: Simultaneous multi-gene profiling and full antigenic mapping across HA (1-16) and NA (1-9) with cost-effective standard PCR equipment.
- Recommended Kits: AIV Multi-tube Kit (IPC11009) for simultaneous multi-target confirmation; HA (IPC12020) & NA (IPC12021.E) Kits for complete phylogenetic lineage identification.

4

Technical Specifications & Ordering Information

iNtRON Biotechnology, Inc.

Global Leader in Molecular Diagnostics & Bacteriophage Therapeutics