iNtRON Biotechnology DR

Life Science (Reagent)

All products
Extraction Reagent
- DNA
- RNA
- AutoPrep
Amplification Reagent
- PCR
- RT/RT-PCR
- Real-time PCR
- Others
Research Products
Instruments & Others

DNA

Life Science (Reagent) Research Products DNA

DNA-spin™ Plasmid DNA Purification Kit

Cat.No	Capacity	Inquire
17096	50 col.
17098	200 col.

Inquire List

Description
TroubleShooting Guide
Related products
Protocol
MSDS

PRODUCT INFORMATION

Description

Spin column type of product for separation/purification up to 20ug of high purity Plasmid DNA

• Up to 20ug high yield and high purity of plasmid DNA can be obtained by separation and purification with high reproducibility.
• Separation and purification for up to 30 kb of plasmid DNA
• Containing Lysis & Neutralization indicator (Lysis Viewer)

DNA-Spin™ Plasmid DNA Purification Kit is able to perform separation and purification for high concentrated, purity plasmid DNA from bacteria using silica membrane and optimized alkali lysis buffering system. Neutralization buffering system used for pH neutralization and binding perform not only separation/purification for plasmid DNA over 30Kb, but also remove chromosomal DNA, cell debris effectively after lysis. Also, pH change through alkaline lysis method can be observed visually in real time because the product contains lysis & neutralization indicator such as Lysis Viewer (Figure 1). Product is used for either High-copy plasmid DNA or low-copy plasmid DNA and in order to perform separation and purification effectively from endA+ strain(HB101, the JM strains, NM series strains, PR series strains, etc.), Washing Buffer A is provided separately.

Figure1. Changes observed by Lysis Viewer

Applications

01Ligation & transformation
02PCR & Real-time PCR
03Microinjection
04Transfection
05Automated fluorescent
sequencing
06Restriction digestion etc.

Kit Contents

Contents	50 COLUMNS	200 COLUMNS
Resuspension Buffer	15ml × 1 ea	55ml × 1 ea
Lysis Buffer	15ml × 1 ea	55ml × 1 ea
Neutralization Buffer	20ml × 1 ea	80ml × 1 ea
Washing Buffer A	30ml × 1 ea	140ml × 1 ea
Washing Buffer B(Concentration)	10ml × 1 ea	40ml × 1 ea
Elution Buffer	20ml × 1 ea	20ml × 1 ea
Spin Column & Collection tube (Yellow color)	50 Columns & tubes	200 Columns & tubes
RNase A (Lyophilized powder)	9mg/ml × 1 tube	33mg/ml × 1 tube
Lysis Viwer	60 µl	220 µl
Manual	1 ea	1 ea

Technical Data

Observation based on effects of Plasmid Copy No.

After extracting plasmid DNA from high copy number and low copy number using DNA-spinTM Plasmid DNA Purification Kit, the results verified high purity and high yield of plasmid DNA.

DH5a/pUC18(High-copy number), DH5a/pET40b(Low-copy number)
iNtRON; Supplier A, Competitor A; Supplier B, Competitor B

Data Comparison for extraction efficiency based on size of plasmid DNA

After extracting various size of plasmid DNA using DNA-spin^TM Plasmid DNA Purification Kit, the results verified higher yield compare to competitors.

Plasmid DNA Size : 2.7 Kb, 6.4 Kb, 9 Kb, 12.5 Kb, 33.4 Kb
iNtRON; Supplier A, Competitor A; Supplier B, Competitor B

Citation List

1	J. Microbiol. Biotechnol. (2010), 20(2), 350–35 doi: 10.4014/jmb.0907.07029 First published online 21 November 2009	Simple Purification of the Human Antimicrobial Peptide Dermcidin (MDCD-1L) by Intein-Mediated Expression in E. coli	Hong, Inpyo, Yong-Seok Kim ,and Shin-Geon Choi	Korea
2	Appl. Environ. Microbiol. 2013, 79(2):610.	Functional Complementation and Effects on Carotenoid Profiles in Escherichia coli	Gyu Hyeon Song, Se Hyeuk Kim, Bo Hyun Choi, Se Jong Han and Pyung Cheon Lee	Korea
3	Carbohydrate Polymers Volume 99, 2 January 2014, Pages 39-46	Transglycosylation of engineered cyclodextrin glucanotransferases as O-glycoligases	Chao Li a, Hee-Jeong Ahn, Jin-Hyo Kim , Young-Wan Kim	Korea
4	Biocheimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Volume 1851, Issue 3, March 2015, Pages 248-260	The capacity for long-chain polyunsaturated fatty acid synthesis in a carnivorous vertebrate: Functional characterisation and nutritional regulation of a Fads2 fatty acyl desaturase with Δ4 activity and an Elovl5 elongase in striped snakehead (Channa striata)	Meng-Kiat Kuah, Annette Jaya-Ram, Alexander Chong Shu-Chien	Malaysia
5	Acta Vet. Brno 2014, 83: 281-286 https://doi.org/10.2754/avb201483040281	Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey	Zafer Ata, Artun Yibar, Erdem Arslan, Kaan Mustak, Elcin Gunaydin	Turkey
6	Volume111, Issue7 July 2014 Pages 1374-1384	Engineering of a butyraldehyde dehydrogenase of Clostridium saccharoperbutylacetonicum to fit an engineered 1,4‐butanediol pathway in Escherichia coli	Hee Jin Hwang, Jin Hwan Park, Jin Ho Kim, Min Kyung Kong , Jin Won Kim, Jin Woo Park, Kwang Myung Cho ,Pyung Cheon Lee	Korea
7	The Journal of Horticultural Science and Biotechnology, Volume 90, 2015 - Issue 4	Carrot (DaucuscarotaL.) heat shock protein 70 gene (DcHsp70) confers tolerance to heat or cold stress in E. coli cells	Eunhye Ko, Hanseul Park,Yeh-Jin Ahn	Korea
8	Journal of Biomedical Nanotechnology, Volume 10, Number 4, April 2014, pp. 571-579(9)	Substrate-Mediated Delivery of MicroRNA-145 Through a Polysorbitol-Based Osmotically Active Transporter Suppresses Smooth Muscle Cell Proliferation: Implications for Restenosis Treatment	Muthiah, Muthunarayanan; Islam, Mohammad Ariful; Cho, Chong Su; Hwang, Jun Eul; Chung, Ik-Joo; Park, In Kyu	Korea
9	Published online before print October 23, 2014, doi: 10.1530/REP-14-0403	Identification and differential expression patterns of porcine OCT4 variants	Jae Yeon Hwang, Jong-Nam Oh, Dong-Kyung Lee, Kwang-Hwan Choi, Chi-Hun Park	Korea
10	Colloids and Surfaces B: Biointerfaces Volume 126, 1 February 2015, Pages 322-327	Formulation of glutathione responsive anti-proliferative nanoparticles from thiolated Akt1 siRNA and disulfide-crosslinked PEI for efficient anti-cancer gene therapy	Muthunarayanan Muthiah, Hui-Lian Che, Santhosh Kalash, Jihoon Jo, Seok-Yong Choi, Won Jong Kim, Chong Su Cho, Jae Young Lee, In-Kyu Park	Korea
11	Accepted manuscript posted online 20 October 2014, doi: 10.1128/IAI.02633-14 Infect. Immun. January 2015 vol. 83 no.1 197-204	Plasmid-Encoded MCP Is Involved in Virulence, Motility, and Biofilm Formation of Cronobacter sakazakii ATCC 29544	Younho Choia, Seongok Kima, Hyelyeon Hwanga, Kwang-Pyo Kimb, Dong-Hyun Kanga and Sangryeol Ryua	Korea
12	EXCLI J. 2015; 14: 601–610. Published online 2015 May 8. doi: 10.17179/excli2014-687	MicroRNA-15b target Sall4 and diminish in vitro UCB-derived HSCs expansion	Mahshid Akhavan Rahnama,Ali Akbar Movassaghpour,Masoud Soleimani, Amir Atashi, Azadeh Anbarlou, Karim Shams Asenjan	Iran
13	Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Volume 1851, Issue 3, March 2015, Pages 248-260	The capacity for long-chain polyunsaturated fatty acid synthesis in a carnivorous vertebrate: Functional characterisation and nutritional regulation of a Fads2 fatty acyl desaturase with Δ4 activity and an Elovl5 elongase in striped snakehead (Channa striata)	Meng-Kiat Kuah a, Annette Jaya-Ram b, Alexander Chong Shu-Chien	Malaysia
14	Rev. Roum. Chim., 2015, 60(7-8), 659-664	ESTIMATION OF RADICAL SCAVENGING CAPACITY OF SOME DIARYL- AND PHENOTHIAZINYL-NITRONES	Hermina PETKES,a Emese GAL,a Cristina BISCHIN,a Iulia LUPAN,b Cornelia MAJDIK,a Castelia CRISTEAa,* and Luminiţa SILAGHI-DUMITRESCUa	Roumania
15	Protein Expression and Purification Volume 116, December 2015, Pages 90-97	Cloning and characterization of a novel thermostable esterase from Bacillus gelatini KACC 12197	Jinyeong Kim, Lili Deng, Eunsoo Hong, Yeonwoo Ryu	Korea
16	2016-12-06 US9512235B2 Grant	RNA-specific binding antibody	Younghoon LeeEuihan JungJung Min LeeInsoo ParkHyo Jeong Hong	Korea
17	Barre, Ramya Smithaveni. Long Island University, The Brooklyn Center, ProQuest Dissertations Publishing, 2016. 10292064.	Interaction between scrambled and inflorescence meristem receptor like kinase 2 in inflorescence stem growth and root epidermal patterning in Arabidopsis thaliana	Su-Hwan Kwak, Joseph Morin, Lorrain Marsh	Korea
18	Jundishapur J Microbiol. 2016 Oct; 9(10): e37897. Published online 2016 Sep 14. doi: 10.5812/jjm.37897	Isolation, Antimicrobial Susceptibility Profile and Detection of Sul1, blaTEM, and blaSHV in Amoxicillin-Clavulanate-Resistant Bacteria Isolated From Retail Sausages in Kampar, Malaysia	Lih-Shin Tew,Li-Yen She and Choy-Hoong Chew	Malaysia
19	Genome, 2017, 60(5): 402-413, https://doi.org/10.1139/gen-2016-0052	Natural variation in CIRCADIAN CLOCK ASSOCIATED 1 is associated with flowering time in Brassica rapa	Hankuil Yi,Xiaonan Li,Seong Ho Lee,Sup Nou, Yong Pyo Lim, Yoonkang Hura	China
20	Front. Chem., 04 July 2018\| https://doi.org/10.3389/fchem.2018.00233	The Sodium Sialic Acid Symporter From Staphylococcus aureus Has Altered Substrate Specificity	Rachel A. , Weixiao Y. Wahlgren, Daniela M. Remus1,2, Mariafrancesca Scalise5, Sarah A. Kessans, Elin Dunevall3, Elin Claesson3, Tatiana P. Soares da Costa6, Matthew A. Perugini6, S. Ramaswamy, Jane R. Allison, Cesare Indiveri, Rosmarie Friemann and Renwick C. J. Dobson	New Zealand
21	Viruses 2018, 10(3), 107; doi:10.3390/v10030107	Recombinant Goose Circoviruses Circulating in Domesticated and Wild Geese in Poland	Tomasz Stenzel , Daria Dziewulska , Brejnev M. Muhire , Penelope Hartnady , Simona Kraberger , Darren P. Martin and Arvind Varsani	South Africa
22	J Vet Sci. 2018 Nov;19(6):759-770. English. Published online Nov 28, 2018. https://doi.org/10.4142/jvs.2018.19.6.759	Hexon and fiber gene changes in an attenuated fowl adenovirus isolate from Malaysia in embryonated chicken eggs and its infectivity in chickens	Norfitriah M. Sohaimi,1 Mohd H. Bejo,1,2 Abdul R. Omar, Aini Ideris and Nurulfiza M. Isa	Malaysia
23	Journal of Genetic Engineering and Biotechnology Volume 16, Issue 2, December 2018, Pages 281-285	Improvement of cellulose degradation by cloning of endo-β-1, 3-1, 4 glucanase (bgls) gene from Bacillus subtilis BTN7A strain	Wafaa K. Hegazy a, Mohamed S. Abdel-Salam a, Azhar A. Hussain b, Hoda H. Abo-Ghalia b, Safa S. Hafez	Egypt
24	Emerg Infect Dis. 2019 Jan; 25(1): 92–101. doi: 10.3201/eid2501.181347	Multiple Introductions of Domestic Cat Feline Leukemia Virus in Endangered Florida Panthers1	Elliott S. Chiu, Simona Kraberger, Mark Cunningham, Lara Cusack, Melody Roelke, and Sue VandeWoudecorresponding author	USA
25	AsPac J. Mol. Biol. Biotechnol. 2022	Recombinant cell penetrating peptides and intrabodies targeting membrane-bound mutated KRAS antigens	Jeremy Jeack Ceen Nga , Zhi Xuan Lowa , Larsen Alessandroa , Jung Yin Fonga , Audrey Onn Yi Au Yongb , Minn-E Ngc , Michelle Yee Mun Teoa , Hok Chai Yama , Lionel Lian Aun Ina*	UK
26	Pak. J. Bot., 55(2): DOI: http://dx.doi.org/10.30848/PJB2023-2(11)	MOLECULAR DISSECTION AND AN IN-SILICO APPROACH OF A NOVEL GIBBERELLIN 20-OXIDASE GENE OF HIBISCUS CANNABINUS L	NOR AINI AB SHUKOR1,2, YUSUF CHONG YU LOK3, SURES M. KUMAR1 , RAMBOD ABIRI1 AND MOHD PUAD ABDULLAH	Malaysia
27	Virology, Volume 565, 2 January 2022, Pages 38-51	Diverse single-stranded DNA viruses identified in New Zealand (Aotearoa) South Island robin (Petroica australis) fecal samples	Joy M.CusteraRobynWhitebHelenTaylorcdKaraSchmidlinaRafaela S.FonteneleaDaisyStaintoneSimonaKrabergeraJames V.BriskiefArvindVarsaniag	USA
28	Iran J Basic Med Sci. 2022 Mar; 25(3): 276–285.	Immunopotentiating properties of chimeric OprF-OprI-PopB protein against Pseudomonas aeruginosa PAO1 in the infected burned rat model	Fattaneh Sabzehali,1 Hossein Goudarzi,1 Mehdi Goudarzi,1,* Alireza Salimi Chirani,1 and Mohammad Hossein Yoosefi Izad1	Iran
29	MDPI, Journals JoF Volume 8 Issue 6 10.3390/jof8060631	Leaf-Associated Epiphytic Fungi of Gingko biloba, Pinus bungeana and Sabina chinensis Exhibit Delicate Seasonal Variations	Lijun Bao 1,2,Bo Sun 2,3,Jiayu Liu 2,Shiwei Zhang 2,Nan Xu 1ORCID,Xiaoran Zhang 4ORCID,Tsing Bohu 5,* andZhihui Bai 2,3,6,*ORCID	China
30	NOR AINI AB SHUKOR ET AL. 2023	MOLECULAR DISSECTION AND AN IN-SILICO APPROACH OF A NOVEL GIBBERELLIN 20-OXIDASE GENE OF HIBISCUS CANNABINUS L.	NOR AINI AB SHUKOR1,2, YUSUF CHONG YU LOK3, SURES M. KUMAR1 RAMBOD ABIRI1 AND MOHD PUAD ABDULLAH	Malaysia
31	National Library of Medicine	Genotyping and molecular investigation of plasmid-mediated carbapenem resistant clinical Klebsiella pneumoniae isolates in Egypt	Kholoud Baraka,1,* Rania Abozahra,1 Marwa Mohammed Haggag,2 and Sarah M Abdelhamid1	Egypt

TroubleShooting Guide

QWhat is the advantage of using DNA-spin Kit?: ADNA-spin Kit provides cap column in order to prevent contamination from other tube during performing centrifugation.

QHow long it takes to extract Plasmid DNA using DNA-spin Kit?: AIt might can different according to experimenter, however, it usually take within 30 min if you follow the instruction.

QWhat is concentration of RNaseA Solution in DNA-spin Kit?: AIt is 9 mg / 33 mg

QWhat is the amount of RNase A before using Resupsension buffer?: AIn case of 50column, add 0.9ml of RNase A solution, in case of 200column, 3.3ml of RNase A solution added into Resuspension Buffer and be sure to store at 4℃ after adding RNase A solution.

QWhat is DNA binding capacity of column and what is the yield?: ADNA binding capacity of the column is 45ug, and the yield is 15~20ug when culture volume is 7ml.

QIs ethanol required to be added into Washing Buffer B?: AIt is a step for removing concentrated salt and it is required to remove salt with EtOH in order to not give damage on next step.

QIs there a solution if RNA gets contaminated?: AExperiment can be performed without any RNA contamination when neutralization is performed.