e-Myco™ plus Mycoplasma PCR Detection Kit
Dried & aliquoted PreMix Kit to detect 209 kinds of mycoplasma including pathogenic mycoplasma
e-Myco™ plus Mycoplasma PCR Detection Kit is Dried & aliquoted PreMix Kit to detect 209 kinds of mycoplasma including pathogenic mycoplasma. Mycoplasma is common and serious contaminants of cell cultures. It has been shown that 30% to 87% of cell cultures are infected with mycoplasma. Many mycoplasma contaminations, particularly in continuous cell lines, grow slowly and do not destroy host cells but are still able to affect various parameters, leading to unreliable or false results. These effects include changes in metabolism, growth, viability, DNA, RNA, and protein synthesis, and morphology. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotechnological products. e-Myco™ plus Mycoplasma PCR Detection Kit is a set of primers designed to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Conventional techniques that are used to detect mycoplasma involve culturing samples on selective media, which needs at least 1 week to obtain results, whereas by performing PCR with this primer set, which is based on conserved 16S rRNA, detection results are obtained in a few hours. The adopted 8-methoxypsoralen (8-MOP) is used to extinguish the template activity of contaminated DNAs. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent interstrand crosslink after photo-activation by incident light at wavelength 320-400nm
Used for the Detection of Mycoplasma species that are most commonly encountered in cell culture
Contents | 8 Tests | 48 Tests |
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e-Myco™ plus Mycoplasma PCR Detection Kit | 8 tubes | 48 tubes |
DNase/RNase-free Distilled Water | 200 ml x 1 vial | 1 ml x 1 vial |
Control DNA | 5 ml x 1 vial | 20 ml x 1 vial |
Manual | 1 ea | 1 ea |
Increased reliability of analysis results through triple checking system
Sample control : a parameter of condition and concentration of template DNA
Target band : a parameter of mycoplamsa infection
Internal control : a parameter of PCR reaction of the detection kit
Detection limit test of e-Myco ™ plus Mycoplasma PCR Detection Kit
To determine the minimal required amount of genomic DNA, genomic DNA was isolated from a pure culture of M. fermentans-infected K562 cells. The isolated genomic DNA was serially diluted for PCR detection. The result indicates that the detection limit with this kit is 10 ~ 20 pg of genomic DNA per test.
Product | Cat.No | Capacity | inquire | ||||
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e-Myco™ Mycoplasma PCR Detection Kit (ver.2.0) |
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SiZer™-100 DNA Marker Solution |
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