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Mycoplasma MDx

e-Myco™ Mycoplasma PCR Detection Kit (ver.2.0)

Cat.No Capacity Inquire
25235 48 tubes Inquire
PRODUCT INFORMATION
Description

Dried & aliquoted PreMix Kit to detect 49 kinds of mycoplasma

  • • 49 kins of Mycoplasma detection
  • • High specificity and sensitivity
       - Hot-start Taq DNA Polymerase applied
       - Primer design with CLP Technology
  • • Effective validation system applied    - Internal Control : It embedded in the product prevents misjudgment that possibly arises from an erroneous PCR test
  • • 8-MOP system
       - 8-MOP solution prevents carryover contamination by PCR products
  • • Dried & aliquoted PreMix, All-in-one system
       - All components pre mixed as dried pellet condition in one tube
       - PCR can be progress after adding Template and primer
       - Gel loading dye premixed to direct electrophoresis
  • • High stability and reproducibility
       - Dried& aliquoted PreMix is control by ALHP system
       - Vacuum packaging can prevent oxidation and humidity

e-Myco™ Mycoplasma PCR Detection Kit(version 2.0) Mycoplasma are common and serious contaminants of cell cultures. It has been shown that 30% to 87% of cell cultures are infected with mycoplasma. Many mycoplasma contaminations, particularly in continuous cell lines, grow slowly and do not destroy host cells but are still able to affect various parameters, leading to unreliable or false results. These effects include changes in metabolism, growth, viability, DNA, RNA, and protein synthesis, and morphology. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotechnological products. The e-Myco™ (ver.2.0) product is a set of primers designed to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Conventional techniques that are used to detect mycoplasma involve culturing samples on selective media, which needs at least 1 week to obtain results, whereas by performing PCR with this primer set, which is based on conserved 16S rRNA, detection results are obtained in a few hours.

The adopted 8-methoxypsoralen (8-MOP) is used to extinguish the template activity of contaminated DNAs. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent interstrand crosslink after photo-activation by incident light at wavelength 320-400 nm. An internal control of this product was constructed to identify false negative results in each reaction. The internal control was designed in such a way that the sample primer pair was used to amplify the internal control and target DNA, which were differentiated by size

Applications

Used for the Detection of Mycoplasma species that are most commonly encountered in cell culture

Kit Contents
Contents 8 Tests 48 Tests
e-Myco™ Mycoplasma PCR Detection Kit(version 2.0) 8 tubes 48 tubes
DNase/RNase-free Distilled Water 200 ml x 1 vial 1 ml x 1 vial
Control DNA 5 ml x 1 viall 20 ml x 1 vial
Manual 1 ea 1 ea
Technical Data

e-Myco™ Mycoplasma PCR Detection Kit(version 2.0) 의 Detection limit test


 

This is the result of checking the detection limit for each sample using e-MycoTM  Mycoplasma PCR Detection Kit (ver.2.0). We checked that the sensitivity was 3.25 pg/ml for template DNA, 12 cells/ml for cell number, and 20 cfu for mycoplasma copy number.

TroubleShooting Guide
QNoTarget band in positive reaction
AIf internal control band is seen, PCR has been performed properly. It is not a problem of the product. If the PCR reaction is inhibited by impurities included in DNA preparation, the use of diluted DNA as a template may be helpful.
QNo internal control band
ACompetition can occur by using high concentrated DNA template. Please repeat the PCR with a diluted template. If the concentration of template is above 50 ng, the signal of internal control may be disappeared by competition with the template.
QPresence of amplified product in the negative control
AD.W. can be contaminated. Perform PCR again with fresh sterile water. Also, we recommend that you use filter tips to reduce contamination and that you use a pipette after sterilization. All procedures should be done in sterilized conditions.
QPoor resolution on agarose gel
AWe recommend to use a 1.5~2% agarose gel. Also, we recommend that electrophoresis is performed for 40 min at 100 V/14 cm using a 6 cm long 2% agarose gel.
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