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Mycoplasma MDx

e-Myco™ Mycoplasma PCR Detection Kit (ver.2.0)

Cat.No Capacity Inquire
25235 48 tubes Inquire

Dried & aliquoted PreMix Kit to detect 49 kinds of mycoplasma

  • • 49 kins of Mycoplasma detection
  • • High specificity and sensitivity
       - Hot-start Taq DNA Polymerase applied
       - Primer design with CLP Technology
  • • Effective validation system applied    - Internal Control : It embedded in the product prevents misjudgment that possibly arises from an erroneous PCR test
  • • 8-MOP system
       - 8-MOP solution prevents carryover contamination by PCR products
  • • Dried & aliquoted PreMix, All-in-one system
       - All components pre mixed as dried pellet condition in one tube
       - PCR can be progress after adding Template and primer
       - Gel loading dye premixed to direct electrophoresis
  • • High stability and reproducibility
       - Dried& aliquoted PreMix is control by ALHP system
       - Vacuum packaging can prevent oxidation and humidity

e-Myco™ Mycoplasma PCR Detection Kit(version 2.0) Mycoplasma are common and serious contaminants of cell cultures. It has been shown that 30% to 87% of cell cultures are infected with mycoplasma. Many mycoplasma contaminations, particularly in continuous cell lines, grow slowly and do not destroy host cells but are still able to affect various parameters, leading to unreliable or false results. These effects include changes in metabolism, growth, viability, DNA, RNA, and protein synthesis, and morphology. Testing for mycoplasma is an essential quality control tool to assure accurate research and reliable biotechnological products. The e-Myco™ (ver.2.0) product is a set of primers designed to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Conventional techniques that are used to detect mycoplasma involve culturing samples on selective media, which needs at least 1 week to obtain results, whereas by performing PCR with this primer set, which is based on conserved 16S rRNA, detection results are obtained in a few hours.

The adopted 8-methoxypsoralen (8-MOP) is used to extinguish the template activity of contaminated DNAs. 8-MOP is known to intercalate into double-stranded nucleic acids and form a covalent interstrand crosslink after photo-activation by incident light at wavelength 320-400 nm. An internal control of this product was constructed to identify false negative results in each reaction. The internal control was designed in such a way that the sample primer pair was used to amplify the internal control and target DNA, which were differentiated by size


Used for the Detection of Mycoplasma species that are most commonly encountered in cell culture

Kit Contents
Contents 8 Tests 48 Tests
e-Myco™ Mycoplasma PCR Detection Kit(version 2.0) 8 tubes 48 tubes
DNase/RNase-free Distilled Water 200 ml x 1 vial 1 ml x 1 vial
Control DNA 5 ml x 1 viall 20 ml x 1 vial
Manual 1 ea 1 ea
Technical Data

e-Myco™ Mycoplasma PCR Detection Kit(version 2.0) 의 Detection limit test


This is the result of checking the detection limit for each sample using e-MycoTM  Mycoplasma PCR Detection Kit (ver.2.0). We checked that the sensitivity was 3.25 pg/ml for template DNA, 12 cells/ml for cell number, and 20 cfu for mycoplasma copy number.



Citation List
Oncotarget. 2015 May 20; 6(14): 12452–12467.

SEL1L SNP rs12435998, a predictor of glioblastoma survival and response to radio-chemotherapy Marta Mellai, Monica Cattaneo, Alessandra Maria Storaci,2 Laura Annovazzi,1 Paola Cassoni,3 Antonio Melcarne,4 Pasquale De Blasio,6 Davide Schiffer,1 and Ida Biunno2,5
2 European Journal of Pharmaceutics and Biopharmaceutics
Volume 88, Issue 3, November 2014, Pages 746-758
Positive-charged solid lipid nanoparticles as paclitaxel drug delivery system in glioblastoma treatment Daniela Chirio a, Marina Gallarate a, Elena Peira a, Luigi Battaglia a, Elisabetta Muntoni a, Chiara Riganti b, Elena Biasibetti c, Maria Teresa Capucchio c, Alberto Valazza c, Pierpaolo Panciani d, Michele Lanotte d, Laura Annovazzi e, Valentina Caldera e, Marta Mellai e, Gaetano Filice f, Silvia Corona f, Davide Schiffer e
3 The Natural Products Journal, Volume 3, Number 1, March 2013, pp. 42-51(10) Anti-Proliferative Activity of Standardized Methanol Extract of Coscinium fenestratum and Its Major Constituent, Berberine, Against Nasopharyngeal Carcinoma Cells  Daker, Maelinda; Yee Lin, Voon; Akyirem Akowuah, Gabriel; Nyet Cheng, Chiam; Nwabueze Okechukwu, Patrick
4 Journal of Cellular and Molecular Medicine,Volume19, Issue9
September 2015
Pages 2162-2171
Histone deacetylase 5 regulates the inflammatory response of macrophages Lukas Poralla , Thorsten Stroh , Ulrike Erben , Marie Sittig , Sven Liebig, Britta Siegmund ,Rainer Glauben
5 Stem cell and development
Volume 24, Issue 9
May 2015
Pharmacokinetics and In Vivo Fate of Intra-Articularly Transplanted Human Bone Marrow-Derived Clonal Mesenchymal Stem Cells Shim Gayong , Lee Sangbin ,Han Jeonghoon , Kim Gunwoo , Jin Hyerim , Miao Wenjun , Yi Tac-Ghee , Cho Yun Kyoung , Song Sun Uk  and Oh Yu-Kyoung


TroubleShooting Guide
QNoTarget band in positive reaction
AIf internal control band is seen, PCR has been performed properly. It is not a problem of the product. If the PCR reaction is inhibited by impurities included in DNA preparation, the use of diluted DNA as a template may be helpful.
QNo internal control band
ACompetition can occur by using high concentrated DNA template. Please repeat the PCR with a diluted template. If the concentration of template is above 50 ng, the signal of internal control may be disappeared by competition with the template.
QPresence of amplified product in the negative control
AD.W. can be contaminated. Perform PCR again with fresh sterile water. Also, we recommend that you use filter tips to reduce contamination and that you use a pipette after sterilization. All procedures should be done in sterilized conditions.
QPoor resolution on agarose gel
AWe recommend to use a 1.5~2% agarose gel. Also, we recommend that electrophoresis is performed for 40 min at 100 V/14 cm using a 6 cm long 2% agarose gel.
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