LiliF Diagnostic ProductsWe will share various news about iNtRON

DNA

i-genomic Plant DNA Extraction Mini Kit

Cat.No Capacity Inquire
17371 50 col. Inquire
PRODUCT INFORMATION
Description

Spin type product for extracting purified DNA from plant specimen as well as processed food

  • • Specialized in extracting DNA from various plant samples such as Gramineae and seed as well as processed food
  • • High reliability and reproducibility by providing optimized 5 types of protocol for each sample
  • • Possible to extract high yield and purified DNA from plant containing secondary product by using Enhancer Solution
  • • Standard sample description and data are provided for more than 100 types of plant specimen and processed food
  • • Concrete instruction is provided to extract DNA easily

The i-genomic™ Plant DNA Extraction Mini Kit extracts genomic DNA more easily and quickly from processed foods such as wheat, high pressure, hot water, juice, and sterilization as well as plant samples such as leaves, stem, roots, fruits and seeds Especially, Gramineae, which has a weak secondary coat compared to other seeds, can be used for extracting plant DNA. It is possible to use PPT Buffer and Enhancer Solution to prevent from interfering with secondary product. It is designed and developed to extract high purified genomic DNA. In addition, since GMO labeling of processed foods using GMO agricultural products is mandatory, this product can extract genomic DNA from plants as well as processed foods. A protocol to extract Genomic DNA from various plant samples was tested using over 100 plant samples to obtain genomic DNA extraction results (see Figure 1) And related data (see Technical Data).

Figure 1. Plant Samples Applied by Standard Protocol

i-genomic™ Series DNA Extraction Mini Kit Kit is specialized for extracting genomic DNA from specific specimen. (Figure 2. Reference). Also, specialized tools such as lysis buffer and inhibitor remover are provided for each sample. Therefore, i-genomic™ Series DNA Extraction Mini Kit is reliable for extracting high yield and purified genomic DNA and provides detailed protocol for high reproducibility. i-genomic™ Series DNA Extraction Mini Kit provides CAPS which packed each column in order to use in pathogen research and minimize contamination. (Figure 3. reference). Therefore, i-genomic™ Series DNA Extraction Mini Kit is applied for not only pathogen research but also SNP genotyping, RAPD analysis, AFLP analysis, RFLP analysis, Microsatellite analysis.

Figure 2. Six kinds of i-genomic™ Series DNA Extraction Mini Kits

Figure림 3. Spin Column & CAPS Information

Applications
  • 01PCR & Real-time PCR
  • 02Southern blotting
  • 03SNP
  • 04Gene cloning
  • 05Genotyping
  • 06Pathogen research etc.
Kit Contents
Contents 50 COLUMNS
Buffer PG (Lysis Buffer) 30ml × 1 ea
Buffer PPT (Precipitation Buffer) 7ml × 1 ea
Buffer PB 50ml × 1 ea
Buffer PWA 40ml × 1 ea
Buffer PWB(Concentration) 10ml × 1 ea
Buffer PE 20ml × 1 ea
Enhancer Solution 0.5 ml x 1 tube
Spin Column(Green color O-ring) 50 Columns
Collection Tube 100 tubes
RNase A, lyophilized 3 mg x 1 tube
Proteinase K, lyophilized 22 mg x 1 tube
Manual 1 ea
Technical Data

Observation for yield and purity of genomic DNA from various plant specimen



Observation for yield and purity of genomic DNA from various plant specimen

TroubleShooting Guide
QIs it possible to use gDNA prep for GMO test in vegetable beverages?
AIt seems to be applicable if you separate the vegetable beverages separately. The appropriate protocol is considered to be applicable to Type E protocol (fruit). However, if applied directly in solution, the lysis itself may be diluted and not be prepped.
QIf you want to extract gDNA from lyophilized leaf, please recommend suitable product.
AApply Type A protocol to i-genomic Plant. At this time, the lyophilized leaves are in the form of powder and do not require any homogenization, so use a small amount (5mg) of sample with little moisture content.
QThe gDNA prep is in progress in dry stem. Since the amount of supernatant is very small after PPT, is not it a product problem?
AGenerally, in fresh stem tissues, 350 ~ 400ul of supernatant is formed after the PPT step, but dried stem has a low water content and about 150 ~ 200ul supernatant is formed. This is a feature of the sample and can be done according to the protocol.
QI would like to extract DNA from sunflower seeds. I want to apply protocol G, but why is it less than other protocols?
AGramineae Seed is weaker than other seeds and contains a lot of secondary products. Since the outer skin is thin and light, adding lysis buffer will float on top of the buffer. In addition, when the lysis process is carried out, the buffer is faded and blown.
QIs not the protocol's binding capacity presented?
AThe loading capacity, DNA binding capacity and elution volume are indicated on the "Notice Before Use" for each part.
Related Product
관련제품 Product,No,Capacity,Inquire list
Product Cat.No Capacity inquire
i-StarTaq™ DNA Polymerase
25161 250 unit Inquire
25162 500 unit Inquire
Maxime™ PCR PreMix (i-StarTaq)
25165 96 tubes (20 ㎕) Inquire
25167 96 tubes x 5 ea Inquire
Maxime™ PCR PreMix (i-StarMAXⅡ)
25281 96 tubes Inquire
i-Taq™ DNA Polymerase
25021 250 unit Inquire
25022 500 unit Inquire
RedSafe™ Nucleic Acid Staining Solution 
21141 1 ㎖ Inquire
SiZer™-100 DNA Marker Solution
24073 0.5 ㎖ Inquire
SiZer™-1,000 DNA Marker Solution
24074 0.5 ㎖ Inquire
SiZer™-1,000 plus DNA Marker Solution
24075 0.5 ㎖ Inquire

List

TOP