LiliF Diagnostic KitsMore Simple, More Accurate, More Effective

Veterinary MDx

LiliF® PAS PCR Kit

Cat.No Capacity Inquire
IP21526 48 tests Inquire
PRODUCT INFORMATION
Background Information

Pasteurella multocida is a Gram-negative, nonmotile, penicillin-sensitive coccobacillus belonging to the Pasteurellaceae family. Strains belonging to the species are currently classified into five serogroups (A, B, D, E, F) based on capsular composition and 16 somatic serovars (1-16). P. multocida is the cause of a range of diseases in mammals and birds, including fowl cholera in poultry, atrophic rhinitis in pigs, and bovine hemorrhagic septicemia in cattle and buffalo. It can also cause a zoonotic infection in humans, which typically is a result of bites or scratches f rom domestic pets. Many mammals (including domestic cats and dogs) and birds harbor it as part of their normal respiratory microbiota. P. multocida causes a range of diseases in wild and domesticated animals, as well as humans. The bacterium can be found in birds, cats, dogs, rabbits, cattle, and pigs. In birds, P. multocida causes avian or fowl cholera disease; a significant disease present in commercial and domestic poultry flocks worldwide, particularly layer flocks and parent breeder flocks. P. multocida strains that cause fowl cholera in poultry typically belong to the serovars 1, 3, and 4. In the wild, fowl cholera has been shown to follow bird migration routes, especially of snow geese. The P. multocida serotype-1 is most associated with avian cholera in North America, but the bacterium does not linger in wetlands for extended periods of time. P. multocida causes atrophic rhinitis in pigs. it also can cause pneumonia or bovine respiratory disease in cattle.

LiliF™ PAS PCR Kit is able to detect directly and specifically Pasteurella multocida by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Pasteurella multocida, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a genetic reagent for animals that is qualitatively detected by polymerase chain reaction (PCR) to detect Pasteurella multocida f rom whole blood, nasal swab and tissues (liver, heart, etc.). In other words, DNase / RNase free water can be rapidly and qualitatively detected by adding a positive control substance, DNase / RNase free water, supplied in the product f rom the DNA extracted f rom the whole blood, nasal swab and tissues (liver, heart, etc.)

• This product is optimized for a general purpose PCR machine with 0.2 ml PCR tube thermal block.

• Based on the Pasteurella multocida genetic database, the reaction products are formed by specific primers of Pasteurella multocida. Use agarose gel to see if a band of target size is detected

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only.

• It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 PAS Detection PreMix 48 tubes
2 PAS Positive Control 25 μl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
TroubleShooting Guide
Related Product
관련제품 Product,No,Capacity,Inquire list
Product Cat.No Capacity inquire
Protocol

List

TOP