Background Information

The major viruses that cause ruminant epidemics include bovine ephemeral fever virus and Bluetongue virus. Both the cow's fever and the bluetongue disease are transmitted through the mosquito of Culicoides. The onset of the fever occurs in the tropical and subtropical regions almost year-round, in the temperate zone from summer to late autumn, and in Korea from late summer to late autumn, Depending on the degree of individual, group or locally develops. Bovine ephemeral fever (Bovine ephemeral fever) is a systemic symptom that fever lasts for 2-3 days and then rapidly falls to normal. Respiratory symptoms caused by transient high fever, emphysema and bronchitis, difficulty in walking due to arthralgia of the mucous membrane, soft palate or limb, and also may lead to miscarriage or stillbirth. In addition, it does not reach our company, but it is a serious economic damage due to a sudden decrease in flow rate with severe fever. Bluetongue is a fever and has symptoms such as inflammation and hemorrhage of the mucous membrane area (ball, lips, face, etc.), hemorrhage, ulceration, and ejaculation due to umbilical cord and malformed fetus. It is a Class A infectious disease designated by the International Bureau as an epidemic. It is impossible to diagnose accurately by clinical symptoms alone, and various diseases and differential diagnosis are needed. Since feces in the early stages of onset contain a large amount of virus, it is necessary to detect viruses or genes directly in the feces or to try virus isolation using cultured cells However, since virus isolation through cultured cells requires a very long time, molecular biology is required for rapid diagnosis.

The LiliF ™ BEFV/BTV RT-PCR Kit was designed to detect very rapidly and accurately the nonstructural proteins of bovine ephemeral fever virus and Bluetongue virus based on a genetic database. RT-PCR, ( Reverse transcription Polymerase Chain Reaction) to amplify only specific genes, which is a very fast and reliable method

Each tube in the kit contains polymerase, reverse transcriptase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR, except templates for these two viruses. It is very easy to add only distilled water and template for RT-PCR.