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LiliF® CPIV RT-PCR Kit

Cat.No Capacity Inquire
IP12072 48 tests Inquire
PRODUCT INFORMATION
Background Information

The parainfluenza virus is similar to the human flu virus, and the parainfluenza virus itself is not dangerous, but easily infects the second infection of a severe respiratory tract infection. Dog parainfluenza virus is transmitted by air infections due to coughing or sneezing of infected puppies, which can also be transmitted through the urine or secretions of infected dogs. This virus is highly contagious, so if you grow several birds together, over 70% will be infected within 1-2 months. If the infection is 7-10 days, the virus will be released through the respiratory tract. Death by Kennel Cough is rare, but it is very painful for dogs and owners. The most common symptoms are dry, rough coughing causes irritation of the larynx, trachea, and respiratory tract, and sometimes a very rough cough, which often exacerbates the neck with excitement, excitement, or pressure. The disease generally affects animals 2 to 8 days after infection, and particularly bad, animals can cause fever and anorexia, while dogs with weak respiratory conditions are at risk for developing pneumonia.

This LiliF ™ CPIV RT-PCR kit can be used for rapid detection of canine parainfluenza virus directly based on a genetic database. RT-PCR (Reverse Transcription-Polymerase) Chain Reaction) is used to amplify only specific genes, which is a very fast and reliable method.

Each tube in the kit contains polymerase, reverse transcriptase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR, except templates for canine parainfluenza virus gene specific amplification. It is very easy to add only distilled water and template for RT-PCR.

 

Principle

• This product is a genetically-detected gene that can be detected by RT-PCR (Reverse Transcription Polymerase Chain Reaction) in order to detect canine parainfluenza virus pathogen from whole blood, lung tissue, tissue, nasal swab and tracheal wash of animal.

• Detection reagent. In other words, the positive control substance, DNase / RNase free water supplied in the product from vial RNA extracted from whole blood, lung tissue, tissue, nasal swab and tracheal wash using viral gene extraction kit can be rapidly and qualitatively detected This product can do.

• This product is optimized for general purpose PCR machine with 0.2 ml PCR tube thermal block. It is based on genetic database of canine parainfluenza virus, so that reaction product is formed by specific primer of parainfluenza virus After completion of the reaction, use an electrophoresis apparatus and agarose gel to check whether a band of a target size is detected.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 CPIV Detection Premix 48 tubes
2 CPIV Positive Control 25 μl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
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