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LiliF® CHV PCR Kit

Cat.No Capacity Inquire
IP11073 48 tests Inquire
PRODUCT INFORMATION
Background Information

Dog Herpes virus is a viral disease that causes miscarriage, stillbirth and premature birth in a pregnant dog and causes sepsis in dogs. The mechanism of the disease is that the virus invades into the body through the mouth or nose, and the virus spreads from the tonsils, the mucosa, the pharynx. It is known to proliferate later in the spleen, liver, lymph nodes, and reticuloendothelial cells. It can be transmitted through the uterus through the uterus, through the fetus during pregnancy, through the acidity during the birth of the fetus, by direct contact with the virus-infected individual, or by contact with the nasal and oral secretions of the infected. In dogs, it spreads through the respiratory and reproductive systems, is susceptible only to dogs, and is known to be affected by the age of infection. The difference in the expression of symptoms according to age is related to temperature. The temperature of newborn puppies is 35 ~ 36 degrees, which is the optimal temperature for virus multiplication. When the temperature rises to 37 degrees, the virus multiplication is suppressed. It is impossible to detect by clinical symptoms alone and it is necessary to detect various diseases and differentiation. Since feces in the early stages of onset contain a large amount of virus, it is necessary to detect viruses or genes directly in the feces or try to isolate viruses using cultured cells However, since it takes a very long time to isolate virus through cultured cells, molecular detection method is required for rapid detection.

LiliF™ CHV PCR Kit was designed to detect the nonstructural proteins of non-structural proteins of the herpes virus directly based on the genetic database and to diagnose it very quickly and accurately. Using PCR (Polymerase Chain Reaction) within 2 hours, It is characterized by its ability to amplify only specific genes, making it a very fast and reliable method.

Each tube in the kit contains polymerase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR, except for template specific amplification for PCR. It is very easy to add only distilled water and template.

 

Principle

• This product is an animal gene detection reagent which detects qualitatively by polymerase chain reaction (PCR) in order to detect dog herpes virus pathogen in animal nasal cavity, conjunctival secretion, swab.

• In other words, it is a product that can be detected qualitatively quickly by adding positive control substance, DNase / RNase free water, supplied from the vial DNA extracted from viral DNA extraction kit from animal nasal and conjunctiva’s secretions and swabs.

• This product is optimized for general purpose PCR machine with 0.2 ml PCR tube thermal block. Based on genetic database of canine herpes virus, reaction product is formed by specific primer of herpes virus, After completion, check whether the target size band is detected using the electrophoresis device and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 CAV Detection Premix 48 tubes
2 CAV Positive Control 25µl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
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