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LiliF® CPV PCR Kit

Cat.No Capacity Inquire
IP11074 48 tests Inquire
Background Information

Dog parvovirus infection is a contagious disease that has been reported worldwide since 1987. Clinically or pathologically, it can be divided into two types, the heart type seen in young puppies, 3 to 8 weeks old, and the enteritis type seen in after 8 week’s dogs. Enteritis is a major symptom of severe vomiting, hemorrhagic diarrhea, dehydration, and leukopenia, with a high mortality rate. Clinical symptoms and pathologic findings are similar to those of cat leukopenia. Treatment for this is known to be effective for early supply of water. The main symptom is severe after 5 days of oral infection, and when susceptible dog is exposed to the virus, 100% of the dogs are exposed to the virus. Approximately 75% of infected infections, 25% of infectious infections, and high mortality can have clinical symptoms. Early symptoms are lack of energy, cause anorexia and vomiting, diarrhea is observed 24 to 48 hours after the onset of vomiting, and diarrhea with dehydration, weight loss, hydrops and blood is observed when continued. Antibodies in serum can be measured 5 days after oral infection, and are highest in 7-8 days. Previously infected or maternal antibodies to canine parvovirus can be determined by measuring the peroral infectious disease. Currently, dog parvovirus is prevalent. Especially, in case of group breeding, the positive rate tends to be high and also the infectivity is high. Generally, the incidence is low compared to the positive rate of the antibody, and it is thought that most of it passes through the infection. In the non-polluted area, when the dog parvovirus invaded, the viral infectivity was strong, and the intestinal type occurred regardless of the age of the dog and showed high mortality rate. Once the dog was infested, all dogs showed the positive rate of antibody. There is a tendency to concentrate on lost puppies (7-14 weeks). It is impossible to diagnose by clinical symptoms alone and various diseases and differential diagnosis are needed. Since feces in the early stages of onset contain a large amount of virus, it is necessary to detect viral or gene directly in feces or try to isolate virus using cultured cells However, since it takes a very long time to isolate virus through cultured cells, molecular biology test is required for rapid testing.

LiliF™ CPV PCR Kit was designed to detect the nonstructural proteins of non-structural proteins of the pavo virus directly based on the genetic database and to diagnose it very quickly and accurately. Using PCR (Polymerase Chain Reaction) within 2 hours, It is characterized by its ability to amplify only specific genes, making it a very fast and reliable method. Each tube in the kit contains polymerase, dNTPs, 10x reaction buffer, RNase inhibitor, specific, primers for detection, and tracking dye for hot start PCR, except for.


• This product is an in vitro diagnostic reagent for animal which is qualitatively detected by polymerase chain reaction (PCR) in order to detect dog parvovirus pathogen in whole blood of animal.

• In other words, it is a product that can be checked qualitatively quickly by adding DNase / RNase free water, which is a positive control substance supplied in the product, from viral DNA extracted from the whole blood of an animal by using a virus gene extraction kit.

• This product is optimized for universal PCR machine with 0.2 ml PCR tube thermal block. Based on genetic database of canine parvovirus, it is possible to produce reaction product by specific primer of parvovirus, After completion, check whether the band of target size is detected by using the electrophoresis device and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 CPV Detection Premix 48 tubes
2 CPV Positive Control 25 µl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
TroubleShooting Guide
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