Background Information

Chicken anemia virus is the cause of chicken anemia. When the infection becomes severe, the immune system such as anemia symptom and thymus is weakened and immune ability is decreased. It is known that damage occurs only in young chicks within 2 weeks of age. In the past, it was called an infectious anemia virus. It is a virus belonging to the circovirus of short-chain circular DNA structure and has a unique nucleic acid structure that is not common in animals. Chickens are susceptible to infection at all ages, but there are no specific clinical symptoms in the sex system and clinical symptoms only occur when infected with young chicks within 2 weeks of age. It is known that infection from horizontal infection from infectious system and infection from contaminated household facility is mainly possible, but it is also possible to infect the nervous system. major infections of egg transmission occur only in about 7% of the infected mother line and the frequency of the egg transmission is low, but the infected individuals die within two weeks after hatching. Infected chickens appear pale in the mucous membranes, hair follicles, and legs 10 days after infection, become weak and weakened, and anemia symptoms are severe. When infected at a young age, the disease occurs between two weeks and three weeks after infection, up to 50%. The survival system then recovers quickly, and when symptoms develop, there is no respiratory or neurological symptoms. Autopsy findings of dead animals indicate that the liver is heavily poured and occasionally white spots appear. The pericardium is heavily infused with fluid, duller, and sometimes accompanied by. The subcutaneous and muscular bleeding is severe, the thymus is severely atrophied, the F capsule is atrophy, and the marrow has a yellowish white color. Chickens have an early infection of chicken anemia, and the recovered chickens have a diminished immune function, mainly cellular immunity. Chickens infected with this disease are susceptible to staphylococcal or clostridial infections due to a decrease in their immune competence and often cause gangrenous dermatitis. It is impossible to diagnose by clinical symptoms alone and various diseases and differential diagnosis are needed. Since feces in the early stages of onset contain a large amount of virus, it is necessary to detect viral or gene directly in feces or try to isolate virus using cultured cells However, since it takes a very long time to isolate virus through cultured cells, molecular biology test is required for rapid testing.

LiliF™ CAV PCR Kit was designed to detect the nonstructural proteins of non-structural proteins of the herpes virus directly based on the genetic database and to diagnose it very quickly and accurately.Using PCR (Polymerase Chain Reaction) within 2 hours, It is characterized by its ability to amplify only specific genes, making it a very fast and reliable method.Each tube in the kit contains polymerase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR, except for template specific amplification for PCR. It is very easy to add only distilled water and template.