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LiliF® ASP PCR Kit

Cat.No Capacity Inquire
IP41001 48 tests Inquire
PRODUCT INFORMATION
Background Information

Aspergillosis is a non contagious disease of a number of animal species caused by fungi of the genus Aspergillus (usually A. fumigatus). Other potentially pathogenic species are A. flavus, A. nidulans, A. deflectus, A. niger and A. terreus. These fungi occur widely in nature and the usual mode of infection is by inhalation with the production of granulomatous lesions and nodules in the respiratory tract from which there may be dissemination to other tissues and organs. Aspergillosis is a fungal infection that develops in the nose and sinuses of dogs and cats. In some areas of the country these infections can be fairly common. Infections with this fungus show very characteristic symptoms including nasal discharge. Aspergillosis can be difficult to diagnose and treat. Early detection and treatment are very important for controlling this potentially damaging disease. There are three symptoms that are characteristic of Aspergillosis: (1) A profuse, clear to opaque discharge from the nostrils that may alternate with episodes of nose bleeding. (2) Ulcerations on the external part of the nose. (3) Pain or discomfort in the nose or facial area. One, two, or three of these symptoms are usually present in infections with Aspergillus.

LiliF™ ASP Kit is able to detect directly and specifically Aspergillus spp. by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Aspergillus spp., allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative Aspergillus spp., the reaction product is formed by specific primers of Aspergillus spp. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 ASP Detection Premix 48 tubes
2 Positive Control 25 μl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
TroubleShooting Guide
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