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LiliF® BRU spp PCR Kit

Cat.No Capacity Inquire
IP21041 48 tests Inquire
Background Information

Brucella is a genus of Gram-negative bacteria which are small (0.5 to 0.7 by 0.6 to 1.5 μm), non-motile, encapsulated coccobacilli.Brucella is the cause of brucellosis, a true zoonotic disease (i.e. human-to-human transmission has not been identified). It is transmitted by ingesting infected food, direct contact with an infected animal, or inhalation of aerosols. Minimum infectious exposure is between 10 - 100 organisms. Brucellosis primarily occurs through occupational exposure (e.g. exposure to cattle, sheep, pigs), but also by consumption of unpasteurised milk products. There are a few different species of Brucella, each with slightly different host specificity. B. melitensis which infects goats and sheep, B. abortus which infects cows, B. suis infects pigs, B. ovis infects sheeps and B. neotomae. Recently new specie was discovered in marine mammals: B. pinnipediae.

LiliF™ BRU spp Kit is able to detect directly and specifically Brucella spp. by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Brucella spp., allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.


• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative Brucella spp., the reaction product is formed by specific primers of Brucella spp. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 BRU spp Detection Premix 48 tubes
2 BRU spp Positive Control 25 μl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
TroubleShooting Guide
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