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LiliF® CLOS PCR Kit

Cat.No Capacity Inquire
IP21073 48 tests Inquire
PRODUCT INFORMATION
Background Information

Clostridium perfringens is one of the most widespread pathogenic bacteria, and has been associated with a range of diarrheal diseases in both humans and animals. In the dog, C. perfringens has been associated with 28~34 percent of diarrheic cases. The most common clinical signs are chronic intermittent or persistent diarrhea. In some animals acute diarrhea is the primary sign. In fact, some of the cases of hemorrhagic gastroenteritis (HE syndrome), characterized by acute bloody diarrhea and an increased packed cell volume that most practitioners have seen over the years, may have been due to Clostridium perfringens enterotoxicosis (CPE). Many animals exhibit signs of large bowel diarrhea, but small bowel signs may be seen as well. In some cases signs may be seen for only a day or two at a time, with persistent recurrences on a weekly, monthly, or on a less frequent basis.

LiliF™ CLOS PCR Kit is able to detect directly and specifically Clostridium perfringens. by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Clostridium perfringens, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative Clostridium perfringens, the reaction product is formed by specific primers of Clostridium perfringens. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 CLOS spp Detection Premix 48 tubes
2 CLOS spp Positive Control 25 μl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
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