Background Information

Leptospirosis is a bacterial disease caused by pathogenic members of the Genus Leptospira. The disease occurs worldwide in numerous animal hosts, including the dog. The canine disease presents as an acute infection of the kidney and liver and, sometimes, as a septicemia. Chronic kidney disease is a common sequel of infection and abortions may occur in pregnant dams. Because many aspects of the infection are poorly understood, there is the possibility that the disease in dogs may go undiagnosed. Leptospira do not multiply outside of the host and their survival depends on environmental conditions in which leptospirae are found, e.g., soil and water conditions. Leptospira are highly susceptible to drying and to pH changes - pH<6 and pH>8 are inhibitory; temperatures < 7~ 10℃ and temperatures > 34~36℃ are detrimental. Leptospira organisms survive up to 180 days in wet soil, for many months in surface water and they survive better in stagnant than in free-flowing water. The source of infection to animals is either by direct contact with infected urine, fetal and placental material or fluids, uterine discharges, or indirect contact from a contaminated environment.

LiliF™ LEPTO PCR Kit is able to detect directly and specifically Leptospira spp. by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Leptospira spp, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.