Background Information

Mycoplasmas are bacteria that lack a cell wall, but are enclosed by a lipid bilayer membrane. They colonize the mucous membranes of the respiratory and genital tracts as well as red blood cells, and are found in many animals and humans. Several species are pathogenic and a variety of infections such as anaemia, arthritis, infertility and respiratory disease have been attributed to infection with mycoplasmas. Mycoplasmas are approximately 0.3–0.8 um in size and readily pass through 450 and 300 nm membranes (Razin and Tully, 1995). They are fastidious in nature and some require cholesterol, sterol or urea for growth. Most Mycoplasma spp. normally appear in the upper respiratory tract. They have also been isolated at necropsy from lungs of dogs and cats with various types of pneumonia but are not generally present in the lungs of healthy animals, especially cats. Also, these are opportunistic organisms normally found in the vaginal canal. In large enough numbers, they may cause infertility, early embryonic deaths, abortion, stillbirths and weak, nonviable pups.

LiliF™ Myco-spp PCR Kit is able to detect directly and specifically Mycoplasma by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Salmonella, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.