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LiliF® SAL PCR Kit

Cat.No Capacity Inquire
IP21631 48 tests Inquire
PRODUCT INFORMATION
Background Information

Salmonella is a rod-shaped, motile bacterium - nonmotile exceptions S. gallinarum and S. pullorum, nonsporeforming and Gram-negative. There is a widespread occurrence in animals, especially in poultry and swine. Environmental sources of the organism include water, soil, insects, factory surfaces, kitchen surfaces, animal feces, raw meats, raw poultry, and raw seafoods, to name only a few. Salmonellosis is a bacterial disease caused by the bacterium Salmonella. Many different kinds of Salmonella can make people sick. Most people have diarrhea, fever, and stomach pain that start 1 to 3 days after they get infected. These symptoms usually go away after 1 week. Sometimes, people have to see a doctor or go to the hospital because the diarrhea is severe or the infection has affected other organs. Salmonella infections can result in a variety of illnesses, such as pneumonia, meningitis and septicemia. The most common clinical signs for both reptiles and people include: (1) Extreme diarrhea (2) Vomiting (3) High fever.

LiliF™ SAL PCR Kit is able to detect directly and specifically Salmonella by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Salmonella, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 2-3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.

Principle

• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.

• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative Salmonella, the reaction product is formed by specific primers of Salmonella. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.

Intended Use

• For Research Use Only, Not for use in diagnostic procedures.

• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.

• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.

Kit Contents
No. contents 48 tests/kit
1 SAL Detection Premix 48 tubes
2 SAL Positive Control 25 μl x 3 tubes
3 DNase/RNase Free Water 1 ml x 1 tube
TroubleShooting Guide
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