LiliF® AFB PCR Kit
American foulbrood (AFB, Histolysis infectiosa perniciosa larvae apium, Pestis americana larvae apium), caused by the spore-forming Paenibacillus larvae ssp. larvae (formerly classified as Bacillus larvae), is the most widespread and destructive of the bee brood diseases. Paenibacillus larvae is a rod-shaped bacterium, which is visible only under a high power microscope. Larvae up to 3 days old become infected by ingesting spores that are present in their food. Young larvae less than 24 hours old are most susceptible to infection. Spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Spores will not germinate in larvae over 3 days old. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium will die but not before it produces many millions of spores. Each dead larva may contain as many as 100 million spores. This disease only affects the bee larvae but is highly infectious and deadly to bee brood. Infected larvae darken and die.
LiliF™ AFB PCR Kit Kit is able to detect directly and specifically Paenibacillus larvae by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Paenibacillus larvae, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.
• This product is a qualitative PCR testing product with CLP™ technology which provided flexibility in Tm (melting temperature) of primer design for optimization of reaction condition, and maximizes PCR specificity and sensitivity through the control of non-specific priming.
• This product is optimized for a general purpose PCR machine with a 0.2 ml PCR tube thermal block. Based on the genetic database of the causative bacteria Paenibacillus larvae, the reaction product is formed by specific primers of Paenibacillus larvae. After completion of the reaction, confirm whether a band of target size is detected using an electrophoresis and agarose gel.
• For Research Use Only, Not for use in diagnostic procedures.
• This kit is developed, designed, and sold for research purpose only. It is not intended to be used for human or animal diagnosis of diseases. Prior to using it for other purposes, the user must validate the system in compliance with the applicable law, directives, and regulations.
• This product is research reagent of infectious disease for professional use to restrict the public use for animal diseases.
| No. | contents | 50 tests/kit |
|---|---|---|
| 1 | AFB Detection Premix | 50 tubes |
| 2 | Positive Control | 25 μl x 3 tubes |
| 3 | DNase/RNase Free Water | 1 ml x 1 tube |
| Product | Cat.No | Capacity | inquire |
|---|
