Background Information

Stonebrood (Aspergillosis larvae apium) is a fungal disease caused by Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger. It causes mummification of the brood of a honey bee colony. The fungi are common soil inhabitants and are also pathogenic to other insects, birds, and mammals. The disease is difficult to identify in the early stages of infection. The spores of the different species have different colours and can also cause respiratory damage to humans and other animals. When bee larvae take in spores, they may hatch in the gut, growing rapidly to form a collar-like ring near the larval heads. After death, the larvae turn black and become difficult to crush, hence the name stonebrood. Eventually, the fungus erupts from the integument of the larvae and forms a false skin. In this stage, the larvae are covered with powdery fungal spores. Worker bees clean out the infected brood and the hive may recover depending on factors such as the strength of the colony, the level of infection, and hygienic habits of the strain of bees

LiliF™ SB PCR Kit is able to detect directly and specifically Aspergillus flavus by CLP™ technology and Maxime ™ technology on the basis of a genetic database of target nucleic acid fragments. Therefore, this kit can diagnose very sensitive, fast and accurately. The kit contains a specific primer set for a highly conserved region based on current sequence alignments of Aspergillus flavus, allowing the DNA detection. It can determine the infecting all serotype and accurately and sensitivity detect multiple detection genes at one time using the conventional PCR method, and take only 3 hours for detection. Fast and sensitive detection of pathogen enables patients to get appropriate treatment and prevent the rapid spreading of disease by separating patients immediately.