LiliF® EV Real-time RT-PCR Kit
Enteroviruses are a genus of positive-sense single-stranded RNA viruses associated with several human and mammalian diseases. Enteroviruses are named by their transmission-route through the intestine (enteric meaning intestinal). Serologic studies have distinguished 71 human enterovirus serotypes on the basis of antibody neutralization tests. Additional antigenic variants have been defined within several of the serotypes on the basis of reduced or nonreciprocal cross-neutralization between variant strains. On the basis of their pathogenesis in humans and animals, the enteroviruses were originally classified into four groups, polioviruses, Coxsackie A viruses (CA), Coxsackie B viruses (CB), and echoviruses, but it was quickly realized that there were significant overlaps in the biological properties of viruses in the different groups. Enteroviruses isolated more recently are named with a system of consecutive numbers: EV68, EV69, EV70, EV71, etc. Enteroviruses affect millions of people worldwide each year and are often found in the respiratory secretions (e.g., saliva, sputum, or nasal mucus) and stool of an infected person. Historically, poliomyelitis was the most significant disease caused by an enterovirus, poliovirus. There are 64 non-polio enteroviruses that can cause disease in humans: 23 Coxsackie A viruses, 6 Coxsackie B viruses, 28 echoviruses, and 5 other enteroviruses .Poliovirus, as well as coxsackie and echovirus, is spread through the fecal-oral route. Infection can result in a wide variety of symptoms ranging from mild respiratory illness (common cold), hand, foot and mouth disease, acute hemorrhagic conjunctivitis, aseptic meningitis, myocarditis, severe neonatal sepsis-like disease, and acute flaccid paralysis.
• The RT-PCR kit used in this product was PCR and Taqman chemistry. During the RT-PCR reaction, a reaction product is formed by a primer specific to the UTR gene region of the enterovirus. At the same time, the Taqman probe specific to each gene is degraded to form fluorescence. It is a method to measure fluorescence formed by real-time PCR cycler. By using each gene-specific primer and probe, it shows high specificity compared with the conventional PCR method. It shows excellent sensitivity compared to the existing PCR method by using the Taqman chemistry fluorescence measurement method.
•Detection and classification of enterovirus genes present in nucleic acid samples extracted from swab samples from cerebrospinal fluid (CSF) and stool.
|1||Real-Time RT-PCR mixture||280 μl x 2 tubes|
|2||Primer & Probe mixture||200 μl x 2 tubes|
|3||Positive Control||25 μl x 3 tubes|
|4||DNase/RNase Free Water||1 ml x 1 tube|
|Patho Gene-spin™ DNA/RNA Extraction Kit||