Background Information
It is an infectious disease that is highly contagious to children. It is caused by enterovirus 71 or other enteroviruses. Coxsackie virus A16 is the causative agent of infectious disease.
Enterobacteriaceae, the causative agent of hand-foot disease, include polioviruses, coxsackieviruses, eco-viruses, and other enteroviruses. It has moderate infectivity and is capable of spreading through the nose, throat secretions, saliva, and blisters or by direct contact with the stool of an infected person. It is also the most infectious disease for one week.
For hand-foot-and-mouth disease, the prevalence of hand-foot disease in infant or child care facilities is common during the summer and autumn, and generally tends to increase within the community at the same time. In particular, encephalitis caused by enterovirus 71 can be fatal and there have been cases of encephalitis deaths in 1997 in Taiwan and in Taiwan in 1998. .
Rapid and sensitive detection of enteroviruses (EVs) enables appropriate treatment for patients with EV infection and allows rapid isolation of the disease by rapid isolation and proper infection management.
The LiliF ™ EV VP1 1st RT-PCR Kit was designed to directly detect unstructured proteins in the genetic database based on the genetic database, allowing rapid and accurate amplification. RT-PCR (Reverse Transcription-Polymerase Chain Reaction ) To amplify only specific genes. The LiliF ™ EV VP1 2nd PCR Kit is capable of highly sensitive detection of specific genes using the nested PCR method for primary amplified products.
Each tube in the kit contains polymerase, reverse transcriptase, dNTPs, 10x reaction buffer, RNase inhibitor, specific primers for detection, and tracking dye for hot start PCR except template for genetic specific amplification, It is very easy to add only distilled water and template for PCR or PCR.
Principle
• This product is designed to detect pathogens in suspected specimens (swab samples from cerebrospinal fluid (CSF) and stool) using reverse transcription-polymerase chain reaction (RT-PCR) It is a reagent for qualitative detection by PCR (transcription-polymerase chain reaction). In other words, it is a product that can be qualitatively detected with vial RNA extracted from a suspect specimen of a human body with a virus gene extraction kit. This product is optimized for universal PCR machine. It is based on the genetic database of the genome of the hand and foot, and the reaction product is formed by the specific primer of the human body disease.
Intended Use
•This product is a genetic detection kit that detects qualitative through nested PCR technique, polymerase chain reaction (PCR) and polymerase chain reaction (PCR) in order to detect human pathogen in human body from suspected specimens.