i-genomic Clinic DNA Extraction Mini Kit
Designed for clinical research, it is a high-performance spin-type product that can be applied to extraction of trace amounts of DNA (pg level) present in various kinds of samples.
i-genomic ™ Clinic DNA Extraction Mini Kit is a Clinic DNA Extraction designed to easily and quickly extract genomic DNA from blood, blood, tissues, fixed tissues, microbial cultures, tissue fluids, swabs, Binding Carrier is applied to this product and it is effective not only for the purification of DNA in trace amounts but also for strong Lysis / Binding efficiency, so it can be used for gum, cigarette butts, fingerprints, nails, hair, It is possible to effectively dissolve samples such as hair and bones except hair follicles, which provides optimal results for gene detection studies. This product has superior extraction efficiency compared to competitors' products not only in Korea but also overseas. It uses binding carrier which does not affect down-stream application, so it is easy to use separated DNA. In addition, it is possible to extract DNA of whole blood without separate erythrocyte removal step, and it is able to perform various sample processing performance. In addition, the optimal protocol for extracting each sample is presented so that anyone can easily extract it and obtain reliable.
Table 1.Protocols of i-genomic™ Clinic DNA Extraction Mini Kit
The i-genomic ™ Series DNA Extraction Mini Kit is a specialized extraction product (see Figure 1) that is able to efficiently extract genomic DNA from specific samples. We identify the specific elements of a particular sample and provide specialized tools such as removal of Lysis Buffer and inhibitor for each sample. Therefore, i-genomic ™ Series DNA Extraction Mini Kit products are designed to be able to reproduce highly reproducible samples with specific protocols by extracting genomic DNA with high yield and high purity. In addition, all products in the i-genomic ™ Series DNA Extraction Mini Kit have significantly reduced contamination concerns by using the CAPS method to individually pack the columns used for efficient pathogen research (see Figure 2). . Therefore, the i-genomic ™ Series DNA Extraction Mini Kit is a specialized product that can be applied to various fields such as SNP genotyping, RAPD analysis, AFLP analysis, RFLP analysis
Picture 1. Six kinds of i-genomic™ Series DNA Extraction Mini Kits
Picture 2. Spin Column & CAPS Information
|Buffer CCL (Pre Lysis Buffer)
||17 ml x 1ea|
|Buffer CRL (Lysis Buffer)||22 ml x 1ea|
|Buffer CWA||40 ml x 1ea|
|Buffer CWB||10 ml x 1ea|
|Buffer CE||20 ml x 1ea|
|Spin Column(Violet color O-ring)||50 columns|
|Collection Tube||100 tubes|
|Binding Carrier||0.45 ml|
|RNase A, lyophilized||4 mg x 1 tube|
|Proteinase K, lyophilized||
22 mg x 1 tube
Comparison of detection ability against clinical microorganisms
After extracting gDNA from various biological swabs using the i-genomic ™ Clinic DNA Extraction Mini Kit, we observed the presence of Chlamydia trachomatis and Neisseria gonorrhoeae in the DNA. As a result, we were able to confirm the positive detection rate higher than the products for other companies' fine
Comparison of DNA extraction efficiency for various micro samples
Genomic DNA was extracted from various micro samples (fingerprint swabs, gums, nails, hair, blood swabs, etc.) using the i-genomic ™ Clinic DNA Extraction Mini Kit. Real time PCR analysis of the extracted DNA with primers and probes specific for Rnase P gene showed that the lower Ct value (higher yield compared to competitor's product when using the i-genomic ™ Clinic DNA Extraction Mini Kit) ). The Y axis on the graph represents the relative yield as a reciprocal of the Log 10 Ct value.
STR analysis of DNA extracted from micro samples
Genomic ™ Clinic DNA Extraction Mini Kit was used to extract ordinary cigarette butts and contaminated cigarette butts from microscopic samples, and STR analysis was performed for human identification. PCR amplification products of each sample were appropriately diluted and electrophoresed with an ABI 3130XL Genetic Analyzer to detect and analyze the total of 15 STR loci on the autosomes. As a result, it was found that the genetic test was possible in both general samples and highly contaminated cigarette butts Of DNA can be identified. In other words, high yields and effective removal of inhibitors can be seen from contaminated samples containing trace amounts of genetic material.
Q I am using G-spin Total Kit now and I am using it very well. Now I have to extract the gDNA from the Dried Blood sample, and while browsing the intron homepage, I saw the i-genomic clinic kit. Is it possible to extract gDNA from a coagulated blood sample using this product?
A The i-genomic clinic kit can be used for extraction. The i-genomic clinic kit can be similar to G-spin Total Kit, but it is designed to extract gDNA from micro-samples such as long-term coagulated blood, cigarette butts, fingerprints on cups and buttons, If you need to extract gDNA from a product that can be confidently recommended.
Q I want to know the exact usage of the i-genomic clinic kit.
A The i-genomic clinic kit is an effective product for extracting gDNA from microscopic samples. Especially, it is possible to extract gDNA effectively from microscopic fingerprints on coagulated blood, small number of hair roots, cigarette butts, handles / cups / buttons, and microscopic samples from hospitals. In addition, its i-genomic clinic kit has been used for the forensic science classroom of the medical college, which requires DNA testing through microscopic samples secured for crime scene verification. Especially, it is applied to the field of identification test through DNA test after finding warfare remains It is efficient enough to be able to do.