NO (Nitric Oxide) Plus Detection kit
The NO (Nitric Oxide) Plus Detection Kit provides a reliable method for indirect measurement of nitric oxide (NO) by quantifying nitrite (NO₂⁻) in various biological samples including urine, plasma, serum, culture media, and tissue homogenates.
This product is based on the Diazotization assay (Griess method). Nitrite reacts with sulfanilamide to form a diazonium salt, which subsequently couples with naphthylethylenediamine to generate a colorimetric signal. The intensity of the color correlates with nitric oxide concentration.
Nitric oxide is an important effector molecule involved in macrophage-mediated cytotoxicity, inhibition of mitochondrial respiration, and DNA synthesis. It is also recognized as a regulator of immune response and anti-tumor activity.
▶ Griess method-based detection for stable NO measurement
▶ Two-solution system for simple assay workflow
▶ Quantitative analysis of nitric oxide via nitrite
▶ Includes nitrite standard for ≥13 control tests
01 - Quantitative analysis of total nitric oxide
02 - Research for cell cytotoxicity and immune response
| Components | ≤ 1000 assay |
|---|---|
| N1 buffer (Sulfanilamide in the reaction buffer) | 50 ml |
| N2 buffer (Naphthylethylenediamine in the stabilizer buffer) | 50 ml |
| Nitrite (FW: 69.0) standard, 2 mM | 1 ml x 2 vial |
| Manual | 1 ea |
Schematic protocol and nitrite standard curve according to 9-serial 2-fold dilution method
Schematic protocol and nitrite standard curve according to 6-serial 2-fold dilution method
| Product | Cat.No | Capacity | inquire |
|---|
