e-Myco™ VALiD-Q Mycoplasma qPCR Detection Kit
e-Myco™ VALiD-Q Mycoplasma qPCR Detection Kit is a product that amplifies a specific gene of Mycoplasma through PCR and detects Mycoplasma in real time using the fluorescence of the amplification product-specific probe.
This kit is a fast and simple real-time PCR detection kit that replaces the direct method of mycoplasma negative testing.
• High sensitivity (10 cfu / ml level) as an alternative to the direct method
• High specificity that does not detect other related micro organisms(including 3 strains related to domestic negative test validation)
• Demonstrated the performance to satisfy the negative test of Mycoplasma based on nucleic acid amplification method of domestic, US and European Pharmacopoeia
• Easy handling, minimizing deviation or contamination between experimenters
• Monitoring Mycoplasma Contamination Before, During and After Cell Production.
• Contamination Management of Cell Lines
• TA cloning for Mycoplasma detection in specimens and cell lines
| No. | Kit Contents | Unit |
|---|---|---|
| 1 | 2X qPCR Master Mix Solution | 280 μl x 2 tubes (10 μl / test × 50 tests) |
| 2 | Mycoplasma Detection Solution | 140 μl x 2 tubes |
| 3 | Positive Control (External PC) | 50 μl x 3 tubes |
| 4 | DNase/RNase Free Water | 1 ml x 1 tube |
| 5 | Manual | 1 ea |
• Should be stored below -20ºC
• Stable for 12 months from the manufacturing date
• Stable Within 6 months after opening, within expiry date of the kit
1. High specificity
[Positive control and 6 bacterial DNAs detecting the internal control signal HEX signal]
• In order to confirm the detection specificity of Mycoplasma of this product, amplification was performed on 6 bacterial DNAs related to Mycoplasma and positive control M. hyorhinis, including 3 bacteria from the KFDA.
• MAM hyorhinis was able to confirm that the target signal FAM signal was amplified, and the positive control and 6 bacterial DNAs detected the internal control signal HEX signal.
• This confirms that e-Myco ™ VALiD-Q Mycoplasma qPCR Detection Kit can detect and react specifically to Mycoplasma.
2. High sensitivity to meet Mycoplasma negative testing guidelines
3. Substitute for the Direct Method
• When Mycoplasma was detected by direct incubation using an initial unknown concentration, one or two colonies were observed in samples diluted up to 1/107.
• When applied to this product, it was confirmed that the target signal was detected up to the concentration of 1/107, which was confirmed by the direct method. This confirms that fraudulent tests using this product can effectively replace the direct method.
Q What is Intron's existing Mycoplasma detection product and e-Myco ™ VALiD-Q Mycoplasma qPCR Detection Kit?
A As a proven product, it is a Mycoplasma detection product that has a sensitivity level that can replace the direct culture method with a detection limit of 10 CFU / ml. In addition, most Mycoplasma can be detected up to 70 kinds and have very high specificity.
Q e-Myco ™ VALiD-Q Mycoplasma What extraction methods can be used to effectively detect Mycoplasma in samples using the QPCR Detection Kit?
A I would recommend the DNA method There is. However, in the case of the cell boiling method, the results may vary depending on the proficiency of the experimenter, so we recommend the Genomic DNA method.
Q Negative control PCR was performed and the target signal was detected. In addition, the experiment was performed using Mycoplasma infection samples, but the internal positive control signal was not measured?
A There are many reasons why a target signal can be detected during analytical control PCR. You may suspect contamination of D.W and test equipment. This product has a high sensitivity to Mycoplasma and the target can be detected even with a small amount of contamination. In addition, the internal control signal is intended to identify errors during testing. If no IPC signal is detected, it is recommended to check PCR condition and template concentration and retest.
| Product | Cat.No | Capacity | inquire | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G-spin™ Total DNA Extraction Mini Kit Best |
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