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Real-time PCR

RealMOD™ Probe M² 2X qPCR mix (with UDG)

Cat.No Capacity Inquire
25360.100 100 Rxn Inquire
25360.500 500 Rxn Inquire
25360.1000 1000 Rxn Inquire
PRODUCT INFORMATION
Description

RealMOD™ Probe M² 2X qPCR mix (with UDG) is a TaqMan probe type product that can perform quantitative PCR using cDNA or gDNA. As a product to which the UDG-system is applied, the amplification product is DNA including Uracil, so the false positive can be significantly lowered by decomposing the positive sample by UDG during the second experiment. This product has a hot-start function, so the dimer generation rate is low and the reproducibility and stability are high. In addition, the qPCR results are excellent even in templates with high GC contents, and it is provided as a 2X Master mix type, so the capacity can be adjusted according to the experimenter.

 

It is composed of aqMan Probe type and is a 2X Master mix type product that can prevent real-time PCR false positives with UDG-system.

 

● Minimization of cross contamination through UDG-system application

● Real-time PCR reaction volume can be controlled with 2X Master mix type

● Hot-start function is included to minimize non-specific reactions

● Excellent reproducibility and stability

● Check excellent qPCR results even in templates with high GC contents​ 

Applications
 - Preventing cross/carry over contamination
 - Detection and quantification of DNA and cDNA target
 - Gene-expression analysis
 - Pathogene and quantification​

Kit Contents
ContentVolumes
25360.1001ml
25360.500 / 1000500ml / 1000ml
Product Advantage
1) By applying UDG-system, cross-contamination is prevented.
2) It is a company-produced product, and it has a competitive price compared to OEM products, and production and supply are easy.
3) Excellent results even in multi target qPCR experiments.

4) Excellent product reproducibility and stability.

 

Technical Data

  

 


 

 

TroubleShooting Guide

 

QPipetting error or missing reagent
ACheck the concentrations and storage conditions of the reagents, including primers, template DNA.Repeat the qPCR.

 


QInappropriate concentration of primers
AOptimize primer concentration according to the instructions.

 


QTemplate amount too high
ADo not exceed maximum recommended amount of template.
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