RealMOD™ Probe M² 2X qPCR mix
| Content | Volumes |
|---|---|
| RealMOD™ Probe M² 2X qRT-PCR mix | 1 ml |
| Manual | 1 ea |
Figure 1. Amplification of ASFV plasmid DNA using RealMOD™ Probe M² 2x qPCR mix
ASFV plasmid DNA was serial diluted 1/10 (Top, Top-1, Top-2, Top-3, Top-4 , Top-5).
Amplification of plasmid DNA using RealMOD™ Probe M² 2X qPCR mix on an CFX-96 Real-time PCR system.
Figure 2. Amplification of salmonella plasmid DNA using RealMOD™ Probe M² 2x qPCR mix
Salmonella plasmid DNA was serial diluted 1/10 (Top, Top-1, Top-2, Top-3, Top-4 , Top-5).
Amplification of plasmid DNA using RealMOD™ Probe M² 2X qPCR mix on an CFX-96 Real-time PCR system.
Figure 3. Amplification of listeria plasmid DNA using RealMOD™ Probe M² 2x qPCR mix
Listeria plasmid DNA was serial diluted 1/10 (Top, Top-1, Top-2, Top-3, Top-4 , Top-5).
Amplification of plasmid DNA using RealMOD™ Probe M² 2X qPCR mix on an CFX-96 Real-time PCR system.
- As a result of the real-time PCR experiment, a dynamic range suitable for the dilution factor (1/10) was observed.
- It has been confirmed that the reliability of the standard curve is very high and the PCR efficiency is also excellent.
Q As a result of the experiment, amplification cannot be confirmed.
A First, please check whether there is any problem in the template extraction process. Second, please check if there are any missing contents (primer, probe, 2X qRT-PCR mix) when preparing the PCR mixture. Third, check the experimental conditions by reviewing the primer design and the annealing temperature of the prime.
Q As a result of the experiment, a signal was detected in the NC (negative control).
A First, non-specific reactions may occur due to environmental factors, so please cleanly disinfect the equipment and test table being used before proceeding. Second, non-specific reactions may occur due to experimental factors (contamination by the template to be used), so please make a mixture, dispense negative control first, and then conduct an experiment on the target.
Q Is ROX dye added to the product?
A ROX dye is not added to this product, and if necessary, it should be added after use.
| Product | Cat.No | Capacity | inquire | |||||||
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| Patho Gene-spin™ DNA/RNA Extraction Kit Best |
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| Viral Gene-spin™ Viral DNA/RNA Extraction Kit |
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