LiliF® Salmonella Real-time PCR Kit (ver.2.0)
Salmonella spp. Food microbiological test kit with microbiological test method
Products applied with the Food Standards Microbial Test Method of Salmonella spp.
High real-time PCR equipment versatility of master mix type
Application of UNG system to prevent false positive detection
Implementation of PCR reaction validation through IPC system.
LiliF® Salmonella Real-time PCR Kit (ver2.0) is a product that implements the microbial test method of the Food Standards Code that can specifically detect the invA gene of Salmonella spp. (Revised in April 2022, Food Standards ▶ Article 8. General test method ▶ 4. Microbial test method ▶ 4.26 Real-time PCR method of molecular biological test method for food poisoning bacteria). It is a versatile master mix type product that can detect target genes up to 10 copy/uL and can use various real-time PCR equipment. LiliF® Salmonella Real-time PCR Kit (ver2.0) is a product that maximizes performance improvement and PCR efficiency by optimizing the food industry’s invA-specific primer/probe with its RealMOD™Probe M2 2X qPCR mix (with UDG). Uracil DNA Glycosylase (UDG) was added to verify the reliability and validity of the results, and the UNG system was applied to prevent false-positive results from cross-over contamination by PCR amplicons. Internal positive control (IPC) DNA that does not affect target detection and IPC-specific primers/probes are added to the product to confirm IPC detection regardless of target amplification, so you can check the effectiveness of the PCR reaction.
• This product is a kit for the detection of harmful microorganisms in various foods and livestock products.
• It is a reagent for qualitative analysis by real-time PCR test for the presence of Salmonella typhimurium or Salmonella enteritidis in microorganism culture.
| No. | Contents | 50 Tests/Kit |
|---|---|---|
| 1 | 2X PCR Mix | 560 μl x 1 tube |
| 2 | Salmonella Detection Sol. | 280 μl x 1 tube |
| 3 | Positive Control | 25 μl x 3 tubes |
| 4 | DNase/RNase Free Water | 1 ml x 1 tube |
Performance
● Target nucleic acid detection result invA gene: 10 copy/uL detection limit confirmed
Q : Is the product that reflects the Food Codex's Salmonella microbiological test method?
A : This product is the Food Standards Code (revised in April 2022) ▶ Article 8. General Test Method ▶ 4. Microbial Test Method ▶ 4.26 Molecular Biological Test Method for Food Poisoning Bacteria A. This product reflects the Salmonella test method. The product's target gene and specific primer/probe sequence information are the same as those listed, and the real-time PCR reaction conditions are also the same.
Q : The detection signal is confirmed in the negative control test group.
A : This product is Salmonella spp. This product contains primers and probes that bind to a specific invA gene. If the target amplification signal is confirmed in the negative control solution (DNase/RNase free water) provided in the product, retest in a situation where contamination is suspected.
Q : Target detection is confirmed, but the IPC amplified signal is not.
A : If the concentration of nucleic acid in the sample used (genomic DNA extracted from the culture medium) is high, the reaction of a primer/probe specific to IPC DNA may affect IPC detection due to the competitive reaction of PCR. If target detection is confirmed, PCR is operating normally even if the IPC amplification signal is not confirmed, and it is okay to judge positive for the target gene. (However, if the IPC amplification signal is not confirmed even in the test result of the negative control solution provided in the product, please contact the manufacturer.)
Q : Do I need to use specific real-time PCR equipment to use my product?
A : This product is a master mix type product consisting of 2x PCR mix and a primer/probe mixture (Detection solution). Therefore, it is recommended to completely thaw the product before use and simply centrifuge it before use. After mixing the required amount of PCR reaction solution according to the number of samples required, it is a product that can be used by dispensing it into a tube suitable for real-time PCR to be tested. This product can be used in real-time PCR equipment.
Q : Is pretreatment necessary for test samples? Are there any DNA extraction products recommended by this product?
A : This product is a product that detects using DNA extracted from the Salmonella enriched culture medium, and pre-treatment is essential. The extraction method verified through this product is the Boiling Method method presented in the Food Codex and our automatic nucleic acid extraction system (Miracle-AutoXT Automated Nucleic Acid Extraction system, IMC-NC15PLUS, extraction reagent: AutoXT total gDNA Extraction Kit, Cat No. 17188) -48/96), and the detection of the target gene was confirmed normally through two extraction methods. We recommend that you use our automated nucleic acid extraction system, which allows for simple and easy extraction of target nucleic acids.
| Product | Cat.No | Capacity | inquire | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G-spin™ Total DNA Extraction Mini Kit Best |
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