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Real-time PCR

Life Science (Reagent) Amplification Reagent Real-time PCR

RealMOD™ Probe R² 2X qRT-PCR mix (with UDG)

Cat.No	Capacity	Inquire
25362.100	100 rxn
25362.500	500 rxn
25362.1000	1000 rxn

Inquire List

Description
TroubleShooting Guide
Related products
Protocol
MSDS

PRODUCT INFORMATION

Description

It is composed of TaqMan Probe type, and Real-time RT-PCR (qRT-PCR) can be easily performed using RNA as a template.

The 2X MasterMix products that can prevent false positives with UDG-system

• Minimize cross contamination by applying UDG

• Proceed cDNA synthesis and real-time RT-PCR in a tube using RNA as a template

• Real-time RT-PCR reaction volume control with 2X MasterMix type

• Minimize non-specific reactions by including hot-start function

• Excellent reproducibility and stability against interfering substances

RealMOD™ Probe R² 2X qRT-PCR mix (with UDG) is a ready-to-use product that contains all the necessary reagents for qRT-PCR with UDG(Uracil DNA Glcosylase). The applied UDG system prevents cross/carry over contamination of PCR products. Also, Hot-start function of RealMOD™ Probe R² 2X qRT-PCR mix (with UDG) prevents primer-dimer formation at the low temperature during qRT-PCR procedure. Thus, RealMOD™ Probe R² 2X qRT-PCR mix (with UDG) enables researchers to do accurate and convenient quantitative analysis over a wide range of template RNA concentrations with its high sensitivity and reproducibility.

Applications

- Preventing cross/carry over contamination

- Detection and quantification of RNA target

- Gene-expression analysis

- Pathogene detection and quantification

Kit Contents

Content	Volumes
25362.100	100T
25362.500 / 1000	500T / 1000T

Technical Data

Wide Instruments Compatibility

• Applied BioSystems : QuantStudio™ 12K Flex, ViiA™ 7, 7900HT, 7500, 7700, StepOne™ & StepOne plus™

• Stratagene : MX3000P™, MX3005P™

• Bio-Rad : CFX96™ & CFX384™, iQTM5 & MyiQ™, Chomo4™, Opticon® 2 & MiniOpicon®

• Qiagen : Rotor-Gene® Q, Rotor-Gene® 6000

• Eppendorf : Mastercycler®, ep realplex2 & ep realplex4

• Roche : LightCycler® 480

• Illumina : The Eco™

TroubleShooting Guide

QAs a result of the experiment, amplification could not be confirmed.

AFirst, please review whether there are any problems with the state of the template. Second, when preparing the PCR mixture. Please review whether there are any missing contents (primer, probe, 2X qRT-PCR mix, third primer). Please review the experimental conditions by reviewing the annealing temperature of the design and primer.

QAs a result of the experiment, a signal was detected in the NC (negative control).

AFirst, since non-specific reactions may occur due to environmental factors, the equipment and experiment table being used. Please proceed by cleaning and disinfecting. Second, non-specific reactions may occur due to experimental factors. Therefore (contamination by the template to be used), prepare a mixture, dispense negative control first, and then do an experiment.

QIs ROX dye added to the product?

AROX dye is not added to this product, and if necessary, it must be used after adding. If there is a selection for the reference dye, please proceed with none.