DNA
G-spin™ Total DNA Extraction Mini Kit [IVD]
| Cat.No |
Capacity |
Inquire |
| 17046D |
200 col. |
 |
PRODUCT INFORMATION
INTENDED USE
G-spin Total DNA Extraction Mini Kit is an in vitro diagnostic medical device used to separate and refine nucleic acids of human derived various samples.
INTRODUCTION
G-spin Total DNA Extraction Mini Kit offers a variety of protocols and allows you to extract genomic DNA by selecting the appropriate method for each sample type.
TroubleShooting Guide
- Q. RNase A and Proteinase K contained in the product are in the form of powder. What should be used to dissolve the buffer? What is the concentration after melting?
- A.RNase A and Proteinase K of this product are supplied as "Lyophilized" and dissolved in pure DW. In case of RNase A, it is supplied in 3mg and dissolved in 0.3ml pure DW, the concentration is 10mg / ml. Proteinase K is supplied in 22 mg and is dissolved in 1.1 ml pure DW at a concentration of 20 mg / ml. Experiment with the amount given in the protocol according to the sample. -20 ℃). After receiving the product, spin down the RNase A and Proteinase K tubes before dissolving and then put in pure DW.
- Q. It is called total DNA. Does it mean whole genome extraction kit?
- A. This product is "Total" which means that gDNA can be extracted from most samples such as whole blood, cell, tissue, fixed tissue and Gram (-) bacteria. gDNA extraction from the sample is possible.
- Q. We use the Blood gDNA extraction kit. I used G-spin Blood for the first time and now I am using i-genomic Blood product. i-genomic Blood was easy to process with RBC lysis and cell lysis step at once. How does whole blood lysis of G-spin Total product proceed?
- A. Thank you for using our product. The G-spin Total DNA Extraction Mini Kit consists of "RBC and Cell lysis at once," which users felt convenient. In addition, up-grade of lysis buffer and binding buffer improves inhibitor removal efficiency.
- Q. We want to extract gDNA from paraffin block tissue. Is there an extractable product?
- A. Extraction is possible with G-spin Total DNA Extraction mini kit. It is possible to extract gDNA from paraffin embedded block and formalin fixed tissue. You can check the pre-treatment and extraction method in "16 page - Protocol E" of the supplied product manual.
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