DNA
G-spin™ Total DNA Extraction Mini Kit [IVD]
| Cat.No |
Capacity |
Inquire |
| 17046D |
200 col. |
 |
PRODUCT INFORMATION
Description
Spin type product for extracting high yield and high purified DNA from various specimen such as Whole Blood, Cell, Tissue, Fixed tissue and Gram (-) Bacteria.
- • Possible to extract DNA from various specimens such as Whole Blood, Cell, Tissue, Fixed tissue and Gram (-) Bacteria
- • Possible to extract high yield and purified DNA with upgraded Lysis Buffer and Binding Buffer
- • In case for extracting specimen from Blood, DNA can be extracted with only one buffer without performing RBC lysis separately.
- • Genomic DNA extraction can be done quickly within 20~30 minutes.
- • Optimized protocol for each specimen is provided.
G-spin™ Total DNA Extraction Mini Kit [IVD] is instructed to extract genomic DNA simply and quickly from animal culture cell, blood, animal tissue, Rodent tail, fixed tissue, hair and Gram negative bacteria. This product provides upgraded Lysis Buffer and Binding Buffer compare to conventional G-spin™ Series in order to extract high yield and purified DNA from various specimens but also make possible to extract from blood without any process needed for RBC lysis. Also, it removes inhibitor in order to be used in downstream applications such as PCR and hybridization research. Also, it provides optimized protocol for each specimens (Table 1. Reference), therefore there is very high reproducibility for test results and expedite experiments. G-spin™ Total DNA Extraction Mini Kit verified its performance through testing various types of specimens.
Table 1. Protocols of G-spin™ Total DNA Extraction Mini Kit
Applications
- 01Cancer research
- 02Gram negative bacterial research
- 03Human genetic research
- 04Viral DNA Research
- 05Detection Assay : PCR, real time PCR
- 06DNA hybridization : Southern blotting, microarray
Kit Contents
| Contents |
50 COLUMNS |
200 COLUMNS |
| Buffer CL |
25ml × 1 ea |
90ml × 1 ea |
| Buffer BL |
25ml × 1 ea |
90ml × 1 ea |
| Buffer WA |
40ml × 1 ea |
160ml × 1 ea |
| Buffer WB (Concentration) |
10ml / 5ml |
40ml / 20ml |
| Buffer CE |
20ml × 1 ea |
40ml × 1 ea |
| Spin Column (Green color) & Collection tube |
50 ea |
200 ea |
| RNase A, lyophilized |
3 mg x 1 vial |
3 mg x 4 vial |
| Proteinase K, lyophilized |
22 mg x 1 vial |
22 mg x 4 vial |
| Manual |
1 ea |
1 ea |
Technical Data
1) DNA Recovery from different amount of blood samples
Comparison of DNA Yields of different amount of blood sample with Competitor’s
The whole blood samples were used from 6.25μl to 200μl. The yields of blood genomic DNA were proportionally increased with blood starting amount. G-spin™ Total DNA Extraction Kit shows improved efficiency of DNA extraction from whole blood samples.
2) High quality and quantity of recovered DNA
G-spin Total DNA Extraction Mini Kit delivers buffy coat-like yield and concentration from whole blood.
DNA yield and concentration from blood samples was obtained from 5 individual donors (200μl of whole blood was processed; 3 replicates were performed from each donor).
3) Recovery Table
The genomic DNA extraction results using G-spin Total DNA Extraction Mini Kit were shown high quality and quantity of DNA collected from cultured cells, blood, tissue samples,gram-negative bacteria and biological swab samples.
Determination of yield and purity data of various samples
Quick Guide
TroubleShooting Guide
- Q. RNase A and Proteinase K contained in the product are in the form of powder. What should be used to dissolve the buffer? What is the concentration after melting?
- A.RNase A and Proteinase K of this product are supplied as "Lyophilized" and dissolved in pure DW. In case of RNase A, it is supplied in 3mg and dissolved in 0.3ml pure DW, the concentration is 10mg / ml. Proteinase K is supplied in 22 mg and is dissolved in 1.1 ml pure DW at a concentration of 20 mg / ml. Experiment with the amount given in the protocol according to the sample. -20 ℃). After receiving the product, spin down the RNase A and Proteinase K tubes before dissolving and then put in pure DW.
- Q. It is called total DNA. Does it mean whole genome extraction kit?
- A. This product is "Total" which means that gDNA can be extracted from most samples such as whole blood, cell, tissue, fixed tissue and Gram (-) bacteria. gDNA extraction from the sample is possible.
- Q. We use the Blood gDNA extraction kit. I used G-spin Blood for the first time and now I am using i-genomic Blood product. i-genomic Blood was easy to process with RBC lysis and cell lysis step at once. How does whole blood lysis of G-spin Total product proceed?
- A. Thank you for using our product. The G-spin Total DNA Extraction Mini Kit consists of "RBC and Cell lysis at once," which users felt convenient. In addition, up-grade of lysis buffer and binding buffer improves inhibitor removal efficiency.
- Q. We want to extract gDNA from paraffin block tissue. Is there an extractable product?
- A. Extraction is possible with G-spin Total DNA Extraction mini kit. It is possible to extract gDNA from paraffin embedded block and formalin fixed tissue. You can check the pre-treatment and extraction method in "16 page - Protocol E" of the supplied product manual.
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