Description
RedStar DNA/RNA Loading Dye (6x) : User friendly than EtBr / Safer Alternative to EtBr
Introducing RedStar DNA/RNA Loading Dye (6x) , the innovative solution for your DNA and RNA visualization needs. Designed to replace traditional Ethidium Bromide (EtBr), RedStar offers enhanced safety without compromising on performance.
- • Safer Than EtBr : Non-carcinogenic and eco-friendly, reducing health risks and environmental impact.
- • Superior Performance : Provides clear, sharp bands and is compatible with a wide range of gel electrophoresis applications.
- • Convenient and Ready-to-Use : Pre-mixed with loading buffer, simplifying your workflow and saving you valuable time.
- • Versatile : Works effectively with both DNA and RNA samples, making it a versatile addition to lab.
RedStar™ DNA/RNA Loading Dye (6x) is a novel and safe nucleic acid stain, offering a superior alternative to the traditional ethidium bromide (EtBr) stain for detecting nucleic acids in agarose gels. The fluorescent dye binds to DNA or RNA, allowing for clear and bright visualization under UV light. This makes it easier to track the progress of electrophoresis and analyze the results. The loading dye is mixed with the samples before loading onto the gel. This simplifies the process and ensures that the dye is evenly distributed throughout the sample. The loading dye contains tracking dyes that migrate at different rates during electrophoresis. This helps to monitor the progress of the run and ensures that the nucleic acids are migrating as expected. The dye is supplied in a ready-to-use format, eliminating the need for additional preparation. Simply mix the dye with your sample, load it onto the gel, and proceed with electrophoresis. By using a fluorescent staining reagent-contained DNA/RNA loading dye, researchers can achieve more accurate and efficient visualization of nucleic acids, streamline their workflows, and improve the overall quality of their electrophoresis results.
Intended Use
Technical Data
1) High Resolution and None migration shift
• Comparative Performance analysis of RedStar DNA/RNA Loading Dye (6x) based on stain type.
• When evaluating the performance using a 100bp DNA ladder, RedStar DNA/RNA Loading Dye (6x) demonstrates superior efficacy at low concentrations and low molecular weights compared to gel stain types and loading dyes from other manufacturers.Fig. 1. Gel analysis of serial 100bp ladder compare other products and RedStar DNA/RNA Loading Dye (6x).
RedStar DNA/RNA Loading Dye (6x) has higher sensitivity compared to EtBr at small molecules and low concentrations.
2) Low Migration Shift at High Concentration and High Molecular Weight
• Comparative Performance Analysis of RedStar DNA/RNA Loading Dye (6x) Based on Stain Type.
• Upon evaluation using a 1kb DNA ladder, RedStar DNA/RNA Loading Dye (6x) exhibits exceptional performance with minimal migration shift at high concentrations and high molecular weights, compared to other gel stain types and commercially available loading dyes. This dye maintains superior resolution and stability, ensuring accurate and reliable results for high molecular weight nucleic acids.
Fig. 2. Gel analysis of serial 100bp ladder compare other products and RedStar DNA/RNA Loading Dye (6x).
RedStar DNA/RNA Loading Dye (6x) has higher sensitivity compared to EtBr at small molecules and low concentrations and has enhanced migration pattern compare to other similar type of dye product (supplier A).
3) High Sensitivity and Low Gel Diffusion
• High Sensitivity : Capable of detecting RNA quantities as low as 100ng.
• Low Gel Diffusion : Exhibits minimal diffusion within the gel, particularly for low molecular weight fragments, ensuring precise and reliable detection.
