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FaSTAR-XT PGS DNA/RNA Kit (I)

Cat.No Capacity Inquire
17602-48 48 Tests Inquire
PRODUCT INFORMATION

 

Products Overview

 

The FaSTAR-XT PGS DNA/RNA Kit (I) is designed for efficient and reliable extraction of viral RNA and DNA from various samples. The FaSTAR-XT PGS DNA/RNA Kit (I) utilizes a streamlined process that minimizes contamination risks and maximizes yield, making it suitable for both clinical testing and research applications. 

 

Each cartridge is pre-loaded with the necessary reagents, simplifying the workflow. Users can easily insert samples into the cartridge, which is designed to be compatible with various sample types, including swabs, whole blood, stool and etc. The FaSTAR-XT PGS DNA/RNA Kit is designed to effectively remove impurities and inhibitors, allowing for the extraction of high-quality nucleic acids. One of the key advantages of this kit is its ability to provide rapid results. The extraction process can be completed in under 12 minutes with a rapid protocol, making it ideal for sensitive applications such as research on viral infections. Additionally, the individual cartridge design facilitates handling and reduces the risk of cross-contamination between samples. This kit is compatible with downstream applications such as PCR and qPCR, ensuring that the extracted nucleic acids can be used for a variety of analyses. Its robust performance has been validated across different viral targets, demonstrating high sensitivity and specificity.

 

• High/Low throughput : The well plate type allows for high throughput processing, while the individual type can process 1 or more low throughput samples, so you can test 1 to 48 samples simultaneously.

• Easy and Simple Usage : This product can quickly and easily extract from pathogenic specimens, and can stably extract viral nucleic acids from various specimen types such as swabs, whole blood, or stool. It is dedicated for use with automated nucleic acid extraction equipment that minimizes the possibility of cross-contamination.
• Rapid : Experiments can be conducted without the need for pre-processing or post-processing steps. Furthermore, when a fast protocol is utilized, the experiments can be completed within 12 minutes
• Efficacy : The product allows for effective viral nucleic acid extraction from a wide range of diverse specimens. (swabs, whole blood, stool or urine, etc.)
Analysis Workflow

 


 

 

Technical Data
1) HIGH EFFICIENCY

• Use of FaSTAR-XT PGS DNA/RNA Kit (I), stable amplification results for various viruses and samples.


• The FaSTAR-XT PGS DNA/RNA Kit demonstrated equal performance in both well plate type (W) and individual type (I). The results of verifying the extraction performance using various pathogenic samples with added virus standard materials showed effective virus nucleic acid extraction performance even at high and low concentrations.


2) HIGH ACCURACY / UNIFORMITY

• FaSTAR-XT PGS DNA/RNA Kit (I) is evaluated using a variety of samples and shows similar or superior performance at high and low concentrations compared to other products and manual type products. It ensures reliable results by maintaining accurate and uniform extraction performance.



Comparative results of the extraction performance of Pestivirus using the FaSTAR-XT PGS DNA/RNA Kit (I) and other products.

FaSTAR-XT PGS DNA/RNA Kit (I) effectively extracted viral nucleic acids from samples (Buccal swab, whole blood) spiking the standard material of the enveloped virus, classical swine fever (CSF) virus, and it was confirmed that the nucleic acid extracts from both individual type (I) and well plate type (W) were stably amplified by real-time PCR.


Comparative results of the extraction performance of β-Coronavirus.


The comparative performance results for β-Coronavirus extraction show that the FaSTAR-XT PGS DNA/RNA kit (I) provides stable extraction performance for enveloped viruses. When evaluated using β-coronavirus spike samples (buccal swabs and sputum), the kit demonstrated equivalent extraction performance compared to other products and manual extraction methods, effectively confirming the detection of the virus.



Comparative results of the extraction performance of Rotavirus


The FaSTAR-XT PGS DNA/RNA kit (I) provides stable extraction performance for non-enveloped viruses. The extraction performance was compared for the non-enveloped virus, rotavirus. In the evaluation using rotavirus spike samples (swabs and stool), this kit demonstrated equivalent extraction performance compared to other products and manual extraction methods, effectively confirming the detection of the virus. It delivers accurate test results for both high and low concentration samples.



3) CROSS-CONTAMINATION


• The FaSTAR-XT PGS DNA/RNA Kit (I) is designed to effectively prevent cross-contamination between samples. By using an automated extraction system and special disposable tubes, the risk of external contamination is minimized, ensuring reliable results in research and diagnostics.


Cross-contamination verification test results


Cross-contamination verification test results

Repeated tests using Pestivirus (0.2 TCID50) samples confirmed consistent positive detection and uniform Ct values in the wells with samples, with no detection in non-input wells.



TroubleShooting Guide

 

QLower nucleic acid recovery than expected?
ASample homogenization was incomplete. Incomplete homogenization samples results is loss of yield within particulates and clump of debris. / The starting samples were compromised. Ensure that samples (e.g., for customer-provided internal controls) were collected, shipped and stored according to recommended guidelines. / The FaSTARprep96 Automated Nucleic Acid Extraction System Instrument was set for the wrong method. / Check that the mixing sleeve is properly installed on the plate. Ensure that all plates are installed in the correct orientation before processing. / Check amount and storage conditions of starting materials

 

QPoor amplification?
AParamagnetic particle carryover may cause interference in amplification reaction. Remove particles in Elution Tube by centrifugation.

 

QCross-contamination?
A Avoid splashing when adding a solvent to the plate. To prevent contamination from sample to sample, each sample is equipped with a separate mixing sleeve and is designed to minimize exposure to the melt through rotary mixing operation

 

QProtocol not an option on the instrument?
A Check the software version of the FaSTARprep96 Automated Nucleic Acid Extraction System instrument. If abnormalities are identified, request the manufacturer to reinstall the firmware version containing the Viral DNA/RNA protocol.
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