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Miracle-Femto™ Western Detection System

Cat.No Capacity Inquire
16035 100㎖ Inquire
PRODUCT INFORMATION
Description

Miracle-Femto Western Detection System 


Miracle-Femto Western Detection System is a highly sensitive chemiluminescent substrate designed for the detection of target proteins in Western blot analysis. This cutting-edge substrate offers unparalleled sensitivity, allowing you to visualize even the most low-abundance proteins with exceptional clarity.

    Femtogram-level sensitivityDetect target proteins with remarkable sensitivity, down to the femto-grade range.


    • Broad dynamic range : Achieve a wide linear dynamic range, enabling accurate quantification of target protein levels.


    • 
    Rapid and simple protocol :  The easy-to-use protocol minimizes hands-on time and streamlines your Western blotting workflow.


    • Stable signal : Enjoy a stable, long-lasting chemiluminescent signal, providing ample time for image capture and analysis.

Workflow

Miracle-Femto Western Detection System is a highly sensitive chemiluminescent substrate designed for the detection of target proteins in Western blot analysis. This cutting-edge substrate offers unparalleled sensitivity, allowing you to visualize even the most low-abundance proteins with exceptional clarity.





Technical Data
1) Femto Grade Sensitivity

• Detect target proteins with remarkable sensitivity, down to the femto grade range.




2) Broad dynamic range

• Achieve a wide linear dynamic range, enabling accurate quantification of target protein levels.


3) Rapid and simple protocol

• The easy-to-use protocol minimizes hands-on time and streamlines your Western blotting workflow.


4) Stable signal

• Enjoy a stable, long-lasting chemiluminescent signal, providing ample time for image capture and analysis.

TroubleShooting Guide
QThe background is high. How can I resolve this?
AThis issue might occur if the concentration of the primary or secondary antibody is too high or if blocking is incomplete. Try reducing the antibody concentration or optimizing it through a dot blot. Additionally, increasing the number or duration of washing steps is recommended.


QThe signal is not detected or appears weaker than expected.
AIf the antigen-antibody binding is insufficient, try reducing the antibody concentration or increasing the antibody incubation time. You may also want to adjust the blocking reagent volume, considering the balance between sensitivity and specificity. Additionally, to prevent excessive washing away of proteins from the membrane during the assay, reduce the intensity or frequency of washing steps. Ensure that sufficient reagent volumes are applied throughout the assay.


QCan a buffer containing sodium azide be used?
ANo, sodium azide inhibits the activity of HRP (horseradish peroxidase), so its use should be avoided.


QAre there any storage conditions or precautions to maintain stable performance?
AAll reagents should be stored refrigerated for up to 2 years and must be kept from freezing. Working solutions are stable at room temperature for up to 1 hour but should be protected from direct sunlight and excessive light exposure.
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