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FaSTAR-XT Tissue DNA Kit

Cat.No Capacity Inquire
17603-96 96 Test Inquire
PRODUCT INFORMATION

 

Products Overview

The FaSTAR-XT Tissue DNA Kit is operated by the FaSTARpre96 automatic nucleic acid extraction system equipment and is intended to purify genomic DNA from various tissue samples. The extracted nucleic acids are easily bound to the surface of the magnetic beads contained in the product and eluted using a dedicated buffer system.

The FaSTARpre96 auto-nucleic acid extraction system must have the FaSTAR-XT firmware installed, and the FaSTARpre96 only high-intensity magnetic rod and sleeve are required to run the appropriate protocol.

The nucleic acid purification process can be carried out in a simple manner with minimal manipulation before automatic purification, and the eluted fraction is eluted with high-purity genomic DNA suitable for use in downstream applications such as PCR and real-time PCR. The FaSTAR-XT Issue DNA Kit can process 1 to 96 samples simultaneously. ​

 

Technical Informations

1) Comparative test with Other Company (Tissue DNA)

[Information]
Each tissue sample was repeatedly extracted 3 times and the electrophoresis result was confirmed. As a result, the intact genomic DNA band was confirmed without any band attraction, and it was confirmed that the nucleic acid was effectively purified.


2) Comparative test with Other Company (Additional)​

 

[Information]
The Real-time PCR method was examined using each food microorganism and halal sample (a sample processed with pig ingredients) and showed better extraction performance than other companies. This product provides extraction performance suitable for food microbiological examination and halal examination.



 


[Information]
The electrophoresis results were confirmed by repeatedly extracting each cultured cell and muscle tissue sample 10 times. As a result, an intact genomic DNA band was confirmed without any band attraction, and stable yield and purity were confirmed in repeated experiments, confirming that nucleic acids were effectively purified.
TroubleShooting Guide

 

Q. Lower nucleic acid recovery than expected? 
A. There may be several possible causes. Below are the comments following the situation:
- Sample homogenization was incomplete.
- Incomplete homogenization results in loss of yield within particulates and clumps of debris.
- Refer to the sample preparation instructions in the manual and prepare the sample.
- The starting samples were compromised. Ensure samples were collected, shipped, and stored according to guidelines.
- The FaSTARprep96 was set for the wrong method. Ensure correct method selection (Tissue DNA/Food Safety/Halal Testing).
- Check the mixing sleeve installation and plate orientation.
- Verify the amount and storage conditions of starting materials.

 


Q. Cross contamination?
A. Avoid splashing when adding solvent to the plate. To prevent contamination, each sample has a separate mixing sleeve and is designed to minimize exposure during rotary mixing.

 

 

 

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