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AutoPrep

AutoXT Plant DNA Kit (I) _ Individual Type

Cat.No Capacity Inquire
17604-48 48 Tests Inquire
PRODUCT INFORMATION
Description

The AutoXT Plant DNA Kit (I) is designed for high-efficiency nucleic acid extraction from various plant tissues, including leaves, stems, and seeds.

This kit utilizes an optimized protocol that enhances yield, purity, and reproducibility, making it ideal for plant genomics, molecular biology research, and diagnostic applications.

 

Characteristics

• Enhanced Purification with Pre-treatment Column

• Time-Saving & Streamlined Workflow

• Broad Compatibility

• Maximized Yield & Purity

Application

• Extraction of high-integrity genomic DNA from various plant tissues (leaves, stems, seeds).

• Effective remove of plant-derived contaminants such as polysaccharides and polyphenols.

• Suitable for PCR, qPCR, and next-generation sequencing (NGS).

Kit Contents
No. Kit Contents Unit
1 Prefilled cartridges 8 cartridges/tray X 6 vial
2 Plunger tip 8 ea/unit X 12 vial
3 RNase A (lyophylized) 3 mg/vial X 2 vial
4 Pre-Column 48 ea/unit X 1 vial
5 Pre-Buffer 20 ml/bottle X 1 vial
6 Cartridge rack 2 ea
7 Manual 1 ea
Storage

• Should be stored dry, at room temperature (15–25°C)

• Stable for more than 24 months

• Stable at -20°C for up to 24 months and 20 times frozen-thawing until the kit’s expiration date

Analysis Workflow

Technical Data

1. High Yield & Purity Performance

DNA total yield (µg) and DNA Purity

 

[Higher and more consistent nucleic acid yields across diverse plant samples.]

• The AutoXT Plant DNA Kits exhibit higher nucleic acid yield across various plant samples, including leaves, stems, and seeds.

• As shown in the graphs, AutoXT consistently outperforms competitor kits (Supplier A & Supplier B), yielding significantly greater amounts of DNA.


[Reliable purity maintenance with minimal contamination across all sample types.]

• The purity of the extracted nucleic acids was assessed using A260/A280 and A260/A230 ratios, which indicate contamination levels from proteins and polysaccharides, respectively.
• AutoXT Plant DNA Kits consistently achieves optimal purity values, ensuring minimal interference from contaminants.


 

2. High-Integrity DNA


Evaluation of DNA Integrity Through Electrophoresis and Bioanalyzer Analysis.

 

※ Gel electrophoresis and Bioanalyzer assessments were conducted to evaluate the structural integrity of extracted nucleic acids.

[High Stability]

• DNA exhibited high stability, with DNA Integrety Number (DIN) values ranging from 8.7–8.9 indicating minimal degradation.

• These results confirm that AutoXT efficiently isolates intact DNA, ensuring suitability for sensitive molecular applications.

 

 

3. Broad Compatibility Across Various Plant Tissues


High-quality DNA is successfully obtained across all sample types, demonstrating the kit’s adaptability to different plant species. 

The extracted DNA exhibits strong and distinct bands, confirming its suitability for downstream applications such as PCR and sequencing.


TroubleShooting Guide
QLow DNA yield?
A• Inadequate tissue disruption.
• Tissue has low DNA content.
• Too much starting material.
• Sample material not stored properly.
• The Miracle-AutoXT Nucleic Acid Extraction System Instrument was set for the wrong method.
• Check that Plunger Tips were added to the plates/cartridges.

 

 

QPoor amplification?
AParamagnetic particle carryover may cause interference in amplification reaction. Remove particles in Elution Tube by centrifugation.

 

 


QCross-contamination?
AAvoid splashing when adding lysates to plates/cartridges.

 

 

QPlant method not an option on the instrument.
AFor the Miracle-AutoXT Nucleic Acid Extraction System Instrument, verify that the instrument is in Engineer mode. Verify that the instrument has firmware which includes the Plant DNA method.
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