Life Science (Reagent)We will share various news about iNtRON

AutoPrep

AutoXT Plant RNA Kit (W) _ Well-plate Type

Cat.No Capacity Inquire
17605-96 96 Tests Inquire
PRODUCT INFORMATION
Description

The AutoXT Plant RNA Kit (W) is designed for high-efficiency nucleic acid extraction from various plant tissues, including leaves, stems, and seeds.

This kit utilizes an optimized protocol that enhances yield, purity, and reproducibility, making it ideal for plant genomics, molecular biology research, and diagnostic applications.

 

Characteristics

• Enhanced Purification with Pre-treatment Column

• Time-Saving & Streamlined Workflow

• Broad Compatibility

• Maximized Yield & Purity

Application

• Extraction of total RNA while minimizing degradation from various plant tissues.

• Effective remove of PCR inhibitors such as polysaccharides and polyphenols.

• Suitable for PCR, qPCR, and next-generation sequencing (NGS).

Kit Contents
No. Kit Contents Unit
1 Prefilled well-plate 6 vial
2 Plunger tip 8 ea/unit X 12 vial
3 DNA removal Column 48 ea/unit X 2 vial
4 Lysis Buffer 60 ml/bottle X 1 vial
5 Manual 1 ea
Storage

• Should be stored dry, at room temperature (15–25°C)

• Stable for more than 24 months

• Stable at -20°C for up to 24 months and 20 times frozen-thawing until the kit’s expiration date

Analysis Workflow

Technical Data

1. High Yield & Purity Performance

RNA total yield (µg) and RNA Purity

 

[Higher and more consistent nucleic acid yields across diverse plant samples.]

• The AutoXT Plant RNA Kits exhibit higher nucleic acid yield across various plant samples, including leaves, stems, and seeds.

• As shown in the graphs, AutoXT consistently outperforms competitor kits (Supplier A & Supplier B), yielding significantly greater amounts of RNA.


[Reliable purity maintenance with minimal contamination across all sample types.]

• The purity of the extracted nucleic acids was assessed using A260/A280 and A260/A230 ratios, which indicate contamination levels from proteins and polysaccharides, respectively.
• AutoXT Plant RNA Kits consistently achieve optimal purity values, ensuring minimal interference from contaminants.


 

2. High-Integrity RNA


Evaluation of RNA Integrity Through Electrophoresis and Bioanalyzer Analysis.

 

※ Gel electrophoresis and Bioanalyzer assessments were conducted to evaluate the structural integrity of extracted nucleic acids.

[High Stability]

• RNA exhibited high stability, with RNA Integrity Number (RIN) values from 8.2–8.5, indicating minimal degradation.

• These results confirm that AutoXT efficiently isolates intact RNA, ensuring suitability for sensitive molecular applications.

 

 

3. Broad Compatibility Across Various Plant Tissues


High-integrity RNA is consistently extracted from all sample types, with clear and sharp bands observed across different plant species. 

This finding confirms that AutoXT effectively isolates intact RNA, making it ideal for transcriptomics and gene expression studies.


TroubleShooting Guide
QLow DNA yield?
A• Inadequate tissue disruption.
• Tissue has low RNA content.
• Too much starting material.
• Sample material not stored properly.
• The Miracle-AutoXT Nucleic Acid Extraction System Instrument was set for the wrong method.
• Check that Plunger Tips were added to the plates/cartridges.

 

 

QPoor amplification?
AParamagnetic particle carryover may cause interference in amplification reaction. Remove particles in Elution Tube by centrifugation.

 

 


QCross-contamination?
AAvoid splashing when adding lysates to plates/cartridges.

 

 

QPlant method not an option on the instrument.
AFor the Miracle-AutoXT Nucleic Acid Extraction System Instrument, verify that the instrument is in Engineer mode. Verify that the instrument has firmware which includes the Plant DNA method.
Related Product
관련제품 Product,No,Capacity,Inquire list
Product Cat.No Capacity inquire
Miracle-AutoXT Automated Nucleic Acid Extraction System Best
IMC-NC15PLUS SYSTEM Inquire
AutoXT Plant DNA Kit (I) _ Individual Type New
17604-48 48 Tests Inquire
AutoXT Plant DNA Kit (W) _ Well-plate Type New
17604-96 96 Tests Inquire
AutoXT Plant RNA Kit (I) _ Individual Type New
17605-48 48 Tests Inquire

List

TOP