Ez C-cell E. coli BL21(DE3)
Ez C-cell
E. coli BL21(DE3) is a high-efficiency competent cell product designed for protein expression, created using a chemical modification method. Optimized for subcloning applications, this product offers a transformation efficiency of 10⁶ CFU/µg, allowing users to quickly and accurately clone their desired genes.
Ez C-cell is provided in convenient, aliquoted units, making it easy to use. Each package includes SOC broth for recovery culture, and plating beads are also provided to facilitate easy spreading on agarplates.
This product contains an Enhancer that boosts transformation efficiency, ensuring effective and reliable results.
Ez C-cell E. coli BL21(DE3) is designed to enable users to perform cloning quickly, easily, and consistently. This simpification allows researchers to focus on their protein expression and research goals without getting bogged down in complex procedures.
Ez C-cell excels in various applications, including life science research, recombinant protein production, and enzyme activity studies, delivering outstanding performance. It sets a new standard in protein expression and significantly enhances research efficiency.
• High Transformation Efficiency
• User Convenience
• Inclusion of Enhancer
• Rapid Growth
• Versatile Applications
• Cost Efficiency
• Competent Cells
• SOC Broth
• Plating Beads
• Control Plasmid| No. | Kit Contents | Unit |
|---|---|---|
| 1 | C-cell BL21(DE3) | 30 ㎕/tube x 20 tubes |
| 2 | Enhancer Solution | 500 ㎕/tube x 1 tube |
| 3 | SOC broth | 1.7 ml/tube |
| 4 | Plating bead | 60~70 ea/tube x 2 tubes |
| 5 | Control DNA | 50 ㎕/tube x 1 tube |
| 6 | Manual | 1 ea |
• Should be stored at or below -80°C until the expiration date indicated
• Avoid freeze-thaw cycles and do not reuse
1. Confirmation of Protein Expression in E. coli BL21(DE3)
• Recombinant plasmids encoding DNA polymerases were transformed into Ez C-cell E. coli BL21(DE3), followed by induction of protein expression using IPTG for 4 hours.
• SDS-PAGE analysis revealed a distinct protein band at approximately 63 kDa post-IPTG induction.
• Comparison of Coomassie Brilliant Blue staining before and after induction demonstrated a faint band at this position prior to induction, while a prominent band was observed around 63 kDa following induction.
• These results confirm the potential of Ez C-cell E. coli BL21(DE3) as a suitable host for protein expression.
2. Effect of Spreading Beads
• Ez C-cell products provide Plating beads to assist with spreading after transformation.
• For a single use, 5 to 6 beads are added, and the plate is gently shaken to perform the spreading process.
• Using Plating beads helps to distribute colonies more evenly across the plate, allowing for approximately 1.4 times more colonies to be observed compared to traditional methods using a spreader.
• The product includes enough beads for 20 spreading applications.
| Product | Cat.No | Capacity | inquire | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ez C-cell E. coli DH5α New |
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| DNA-spin™ Plasmid DNA Purification Kit Best |
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| MEGAquick-spin™ Plus Total Fragment DNA Purification Kit New Best |
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| IPTG Solution(0.1M) |
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