Patho Gene-spin™ HS DNA/RNA Extraction Kit
The Patho Gene-spin™ HS DNA/RNA Extraction Kit is designed for rapid and sensitive isolation of DNA and RNA from a variety of pathogen such as virus, bacterium and etc.
Samples can be either fresh or frozen plasma/blood (treated with anticoagulants other than heparin), serum, other cell-free body fluids and pathogen-infected cell/tissue.
One of the main advantages of the the Patho Gene-spin™ HS DNA/RNA Extraction Kit is that it can effectively lysis a variety of samples using Proteinase K in the lysis phase, especially the carrier RNA, which allows for high sensitivity nucleic acid extraction, making it suitable for downstream applications.
The kit quickly and effectively purifies DNA/RNA using silica-gel membrane technology. The usage of this product is so simple that users can purify DNA/RNA from various target sources within 30 minutes.
• Suitable for various pathogens
• High-Sensitivity Extraction
• Simple composition
• Ease of Use
• Pathogen detection
• PCR or RT-PCR
• Quantitative PCR (qPCR, qRT-PCR)
• cDNA syntehesis
• Infectious disease research etc.
| No. | Kit Contents | Unit |
|---|---|---|
| 1 | Proteinase K (Lyophilized) | 6 mg X 1 vial |
| 2 | Carrier RNA (Lyophilized) | 300 µg X 1 vial |
| 3 | Lysis Buffer | 35 ml X 1 bottle |
| 4 | Binding Buffer | 35 ml X 1 bottle |
| 5 | Washing Buffer A | 30 ml X 1 bottle |
| 6 | Washing Buffer B | 10 ml X 1 bottle |
| 7 | Elution Buffer | 20 ml X 1 bottle |
| 8 | Spin Column | 10 columns/blister X 5 packs |
| 9 | Manual | 1 ea |
• The Patho Gene-spin™ HS DNA/RNA Extraction Kit
: Should be stored dry at room temperature (15–25°C), can be stored for up to 24 months without showing any reduction in performance
• The Freeze-dried proteinase K and carrier RNA
: Should be avoided for long-term storage at room temperature. Store at -20°C after opening the product, stabilize until the kit expires (24 months)
• The proteinase K & carrier RNA reconstituted by resuspension in D.W.
: Should be avoided for prolonged use at room temperature
1. Enhancing high-sensitivity extraction performance for a variety of low-titer samples
A. High-efficient extraction of viral RNA (
β-Coronavirus )
The Patho Gene-spin™ HS DNA/RNA Extraction Kit showed high extraction efficiency for a variety of clinical samples for low-titer
β-Coronavirus . In particular, enhanced detection results were found in whole-blood and stool samples.
(Enhanced Cut-off value, >2)
B. High-efficient extraction of Bacterial DNA (
C. chauvoei )
The low-titer samples of the gram-positive bacteria-
C. chauvoei also showed high extraction sensitivity, especially in stool, and a relatively significantly enhanced detection value was observed, indicating strong extraction performance. (Enhanced Cut-off value, >3)
2. Flexible Elution Volume Adjustment
• The elution can be adjusted from 40 ㎕ to 50 ㎕ depending on the desired volume. Generally, 50 ㎕ is recommended, but if you need to increase the nucleic acid concentration to increase the extraction sensitivity, you can use 40 ㎕.
• After extracting viral nucleic acids using pestivirus sample, the detection results was checked by qPCR, indicating an enhanced cut-off value when the elution was 40 ㎕ compared to the results obtained using 50 ㎕.
3. High-sensitivity extraction performance for various pathogenic clinical samples
The Patho Gene-spin™ HS DNA/RNA Extraction Kit has effective in extracting nucleic acids from various samples and shows high-sensitivity extraction performance by minimizing the loss of nucleic acids extracted from low-titer samples.
Provides optimized Extraction performance for variety of pathogenic samples
The extraction test used 3 enveloped viruses (
pestivirus ,
β-Coronavirus , and PRRSV) and 2 non-enveloped viruses (IBDV,
rotavirus ), Gram-positive bacteria (Clostridium sp.), and Gram-negative bacteria (Salmonella sp.) on a low-titer basis, and was performed on 4 spiked biological samples: (I) whole blood, (III) stool, (III) urine, and (IV) tissue.
The Patho Gene-spin™ HS DNA/RNA Extraction Kit showed high-sensitivity extraction from a variety of samples and showed the same or superior extraction performance as other products.
The product has excellent nucleic acid extraction performance in a variety of clinical samples with low nucleic acid content and high extraction difficulty. These results provide optimal results for downstream applications such as molecular biology research.
| Product | Cat.No | Capacity | inquire | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Viral Gene-spin™ Viral DNA/RNA Extraction Kit |
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| Patho Gene-spin™ DNA/RNA Extraction Kit Best |
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