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Protein

Golden-STAIN™ Prestained Protein Ladder

Cat.No Capacity Inquire
24053 500 ㎕ Inquire
PRODUCT INFORMATION
Description

The Golden-STAIN Prestained Protein Ladder is a ready-to-use molecular weight marker designed for accurate protein size estimation during SDS-PAGE and Western blotting. Covering a broad molecular weight range of 11–255 kDa, this ladder provides reliable reference bands for diverse protein analysis applications.

 

Featuring sharp, high-contrast bands and color-coded reference markers, it enables intuitive and rapid band identification without post-staining. Optimized for consistent migration across various electrophoresis conditions, the ladder ensures reproducible and dependable results in every experiment.

 

Key Features

• Broad Molecular Weight Range

Covers a wide range of 11–255 kDa, suitable for comprehensive protein size estimation across various applications.

 

• Distinct Color-Coded Bands

Five color-coded marker bands (Blue, Orange, Purple, Green, Light Green) provide visual anchors for quick and accurate band identification.

 

• Sharp and High-Contrast Band Pattern

Delivers clear and well-defined bands with uniform intensity, enabling precise molecular weight estimation.

 

• Reliable Detection at Low Loading Volumes

Maintains excellent visibility even at low sample volumes, ensuring consistent performance and sensitivity.

 

• Ready-to-Use Convenience

No dilution or preparation required, allowing for simple and efficient workflow integration.

Storage

• Storage Temperature

: 4°C (Short-term, up to 3 months) / -20°C (Long-term storage)

 

• Shelf Life

: 24 months from the date of manufacture (Stable for up to 3 months after opening)

Kit Contents
No. Kit Contents Unit
1 Golden-STAIN™ Prestained Protein Ladder 500 μl × 1 vial
2 Manual 1 ea
Technical Data

1. Clear Band Differentiation with Defined Molecular Weight References

 

• Provides 12 distinct prestained bands covering a wide range of 11–255 kDa

• Four color-coded reference bands (5 colors in total including its base color) serve as visual anchors for rapid and intuitive size estimation

• Enables clear band discrimination during both SDS-PAGE and Western blotting


 

 

 

2. Enhanced Band Clarity and Sensitivity Compared to Previous Product

 

• Demonstrates sharper and more distinct band patterns compared to previous ladder versions

• Maintains uniform color intensity across all bands without signal loss

• Ensures reliable visibility even at low loading volumes (1–5 μl)

• Minimizes background interference, supporting accurate and confident protein sizing


 

 

 

3. Accurate Molecular Weight Reference Across Gel and Buffer Systems

 

• Exhibits consistent migration behavior across various gel concentrations and buffer systems

• Provides reliable molecular weight estimation under different electrophoresis conditions

• Reduces variability caused by experimental conditions, ensuring reproducible and dependable results




TroubleShooting Guide
Q Bands appear faint or unclear during electrophoresis.
A • Insufficient loading volume. Increase loading to 3–5 μl.
• Gel percentage may not be optimal for target size range.
• Electrophoresis running conditions may need adjustment.

 

 

Q Bands are smeared or distorted.
A • Overloading of ladder sample.
• Degraded gel or improper polymerization.
• Running buffer may be exhausted or incorrectly prepared.

 

 

Q Color bands are difficult to distinguish.
A • Insufficient contrast due to low loading volume.
• Gel thickness or staining background may affect visibility.
• Ensure proper electrophoresis conditions for optimal band resolution.

 

 

Q Bands migrate differently than expected molecular weights.
A • Migration patterns may vary depending on gel concentration and buffer system.
• Use appropriate gel percentage for target protein size.
• Compare results with a reference ladder under the same conditions if necessary.

 

 

Q No bands are visible after loading the ladder.
A • Ladder may not have been loaded properly.
• Check pipetting accuracy and loading step.
• Verify storage conditions and ensure the product has not degraded.

 

 

Q Weak signal after Western blot transfer.
A • Insufficient transfer efficiency.
• Reduce transfer time or optimize transfer conditions.
• Ensure appropriate membrane and buffer system are used.
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