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RNA

easy-BLUE™ Total RNA Extraction Kit

Cat.No Capacity Inquire
17061 100 ㎖ Inquire
PRODUCT INFORMATION
Description

easy-BLUE™ Total RNA Extraction Kit is a solution type that can extract total RNA easily and quickly from solid samples such as cell, tissue, and plant etc.

This kit is the product that can most efficiently extract the RNA from tissues or cells. It is theoretically possible to extract pure RNA with almost no contamination of protein and genomic DNA when the amount of RNA extraction sample is appropriate.

 

Characteristics

• Total RNA extraction from solid samples such as cell, tissue and plant etc

• Easy separation of aqueous phase/ phenol phase with deep-cyan blue color solution

• High efficiency and purity total RNA extraction using high purity water-saturated phenol

• Optimized protocol for each specimen

• Completely eliminates cross-contaminants for precise downstream applications
Application

• cDNA synthesis

• RT-PCR

• Real-time RT-PCR

• Northern blotting

• Primer extension

• Infectious disease research

Kit Contents


No. Kit Contents Unit
1 easy-BLUE™ Solution 100ml × 1 ea
2 Manual 1 ea
Storage

• Store in the dark at 4ºC.

Technical Data

1. Total RNA extraction from various tissues and cells


The easy-BLUE™ Total RNA Extraction Kit provides a simple and rapid method for the isolation of total RNA from cultured cells, tissues, plants and numerous other cell sources.

• RNA was extracted from various cells and tissues using the easy-BLUE™ Total RNA Extraction Kit.

• As results, it was confirmed that high-purity RNA could be extracted.


 

2. RT-PCR Amplification
 

 

 

Evaluation of the specificity of the e-Myco™ VALiD-Q Real-time PCR Kit (ver. 2.0) for Mycoplasma related strains.

 

Total RNA was purified from different tissues and cells using easy-BLUE™ Total RNA Extraction Kit.

• And then, one step RT-PCR reaction performed for β-actin (400bp) gene from RNA using ONE-STEP RT-PCR PreMix Kit (Cat. No. 25101), respectively.

 

3. Comparison of total RNA yield from different company kit
 

 

 

RNA was extracted from various cells and tissues using the easy-BLUE ™ Total RNA Extraction Kit.  

• As results, its extraction efficiency showed equal to or better than other products.

Citation List
TroubleShooting Guide
QWe want to extract RNA from plant using easy-BLUE. protocol says that a P-buffer is needed. What is the composition of a P-buffer?
AP-Buffer (for Plant): Final 0.8M sodium citrate 1.2M NaCl] The information can also be found in the 'PREPARING SOLUTION BEFORE USE' section of the product manual. It is also available exclusively for the "easy-spin™ llp Plant RNA Extraction Kit" with the addition of a column for plant RNA extraction.
QI would like to extract RNA from Kidney. I am afraid that RNA degradation might happened while performing homogenization because kidney tissue is very stick. Is there any other possible way to increase recovery rate of RNA?
AFirst of all, try to add liquid N2 after obtaining thin tissue fragment and then perform homogenization to increase yield and purity. Also, please be cautious for repetition of freezing/thawing. Please use 10mg of kidney sample because tissue is very active.
QI see gDNA contamination often. Is there any possible way to minimize gDNA contamination?
AWhile moving aqueous layer, do not try to pipette hardly and pipette only volume of 400ul. gDNA may mix with interface layer when you try to extract large amounts of RNA.
QDoes blue lights from easy-Blue affects purity of RNA and recovery rate?
AThe reason for blue light is because of pH indicator which increase RNA recovery rate. Especially, it gives blue light so it is convenient for separating aqueous layer.
QI was using easy-BLEU and I did not use it for many time but, blue color has changed to black. Can I keep use this kit? does it affects RNA recovery rate?
ABlue light from easy-BLUE is because of pH indicator. color change has happened because pH has changed. Therefore, RNA recovery rate may decrease so we recommend you to use new kit. Also, the reason for color change is that kit might stored at room temperature. Please store kit at 4℃ to obtain high RNA recovery rate.
Related Product
관련제품 Product,No,Capacity,Inquire list
Product Cat.No Capacity inquire
easy-spin™ Total RNA Extraction Kit Best
17221 50 col. Inquire
ONE-STEP RT-PCR PreMix Kit
25101 50 rxn. Inquire
Power cDNA Synthesis Kit 
25011 30 rxn. Inquire

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