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RNA

easy-spin™ Total RNA Extraction Kit

Cat.No Capacity Inquire
17221 50 col. Inquire
PRODUCT INFORMATION
Description

Spin type product which can extract high purity and yield total RNA without any genomic DNA contamination easily and quickly from cells and tissues

  • • A combination product of phenol solution and spin column
  • • Minimization of genomic DNA contamination
  • • High yield and high purity total RNA extraction
  • • Unnecessary of Alcohol PPT procedure because of using column to extract total RNA
  • • Highly reproducible results with the use of column
  • • RNA extraction within 30 minutes

easy-spin™ (DNA free) Total RNA Extraction Kit is designed to extract total RNA from cell and tissue in easy and fast way without mixing of genomic DNA. RNA extraction product is normally divided into column type and solution type. Solution type (phenol use) has higher yield comparing to spin type and also can extract large amount of RNA. Even though alcohol precipitation step is required due to the use of phenol, it has been widely used to extract RNA. On the contrary, spin type does not required alcohol precipitation to extract pure RNA, but it is mainly used for the rapid extraction of RNA from many samples, or for the safety and eco-friendliness of experimenters and the ease of experimentation.

As described above, the RNA extracted in the above two forms should have enough purity and yield to be used in almost all molecular biological experiments such as Northern blot analysis, cDNA synthesis and RT-PCR. It is a easy-spin™ (DNA free) Total RNA Extraction Kit that combines the advantages of the solution type and the column type. This product eliminates the inconvenience of the alcohol PPT stage when using the solution type product, and total RNA can be extracted within 30 minutes without contamination of genomic DNA. This product significantly reduces contamination concerns by using the CAPS method to individually pack the columns to effectively perform pathogen research (see Figure 1).

[ Figure 1. Spin Column & CAPS Information ]

Applications
  • 01Infectious disease research
  • 02RT-PCR and real-time RT
  • 03Northern Blotting
  • 04Dot Blotting
  • 05Primer extension
  • 06cDNA synthesis, etc.
Kit Contents
Contents 50 COLUMNS
Lysis Buffer(easy-BLUETM Solution) 50ml × 1 ea
Binding Buffer 20ml × 1 ea
Washing Buffer A 40ml × 1 ea
Washing Buffer B(Concentration) 10ml × 1 ea
Elution Buffer 20ml × 1 ea
Spin Columns(Orange color) 50 Columns
Collection Tubes 50 tubes
Manual 1 ea
Technical Data

Comparison of extraction efficiency of total RNA according to various product groups

Total RNA was isolated and purified from SNU-1 cells using the easy-spin™ Total RNA Extraction Kit and competitors products; solution type and column type. In the case of extraction using the easy-spin™ Total RNA Extraction Kit, we could see that the extracted total RNA was superior to the total RNA extracted by the competitors products.

Lane M; marker; lane 1, easy-spin™ Kit; lane 2, supplier A(phenol type); lane 3, supplier B(column type)

Results of genomic DNA contamination

We confirmed the presence of gDNA contamination by PCR amplification using IL-10 primer and total RNA extracted from easy-spin™ Total RNA Extraction Kit and competitors products; solution type and column type. In the case of the competitors products, gDNA contamination was confirmed in phenol type and column type, while we could confirm that gDNA contamination was negative in the RNA extracted by the easy-spin™ Total RNA Extraction Kit.

Lane M; marker; lane 1, easy-spin™ Kit; lane 2, supplier A(column type); lane 3, supplier B (phenol type); lane P, control template gDNA

 

 

Citation List

 

 

1 Annals of the Romanian Society for Cell Biology, 8092–8106 The Potential Role of Vitamin D Supplementation in Ameliorating the Pathogenesis of Induced Type 2 Diabetes in Rats Mona I. Abdel Ati, Heba F. Pasha, Amal M. El-Gayar, Mamdouh M. El-Shishtawy Iraq
2 Wiley Online Library, 27 June 2022 https://doi.org/10.1111/are.15963 Validation of reference genes for gene expression analysis in melanin-injected black tiger shrimp (Penaeus monodon) Hong-Nhung T. Phan,Hong-Loan T. Nguyen,Ha-Giang Vu,Dinh-Thang Nguyen,Thai Nho Dinh,Lua T. Dang,Trang Thi Ngo,Le-Na T. Nguyen,Tuan-Nghia Phan Vietnam
3 Life Sciences, Volume 297, 15 May 2022, 120443 Antitumor effects of rhamnazinon sorafenib-treated human hepatocellular carcinoma cell lines via modulation of VEGF signaling and PI3K/NF-κB p38/caspase-3 axes cross talk Yasmin H.HabibaaGamal A.OmranaMaged W.HelmybMaha E.Houssena Egypt
4 Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1864, Issue 11, 1 November 2022, 184014 Activation of serotonin receptor 2 by glucosylsphingosine can be enhanced by TRPA1 but not TRPV1: Implication of a novel glucosylsphingosine-mediated itch pathway RamshaAfzalaWon-SikShimbc Korea
5 Research Square, March 22nd, 2022, DOI: https://doi.org/10.21203/rs.3.rs-1420695/v1
Functional Relationship of Interferon Regulator Factor-5, Interferon-γ,
and Hypoxia-Induced Factor-1α with COVID-19 During Pregnancy
Outcomes
Dilsad Herkiloglu, Omer Erdogan, Erkan Gumus, Ozge Cevik, Sarfraz Ahmad
USA
6 Springer, Medical Oncology volume 39, Article number: 143 (2022) Unraveling the therapeutic potential of GANT61/Dactolisib combination as a novel prostate cancer modality Mohamed Youssef, Nermine Moussa, Maged W. Helmy & Medhat Haroun  Egypt
7 Applied Animal Behaviour Science
Volume 261, April 2023, 105902
Different LED light colors modify behavior, physiology, and hypothalamic CRF and NPY mRNA expression in Japanese quail (Coturnix coturnix Japonica) Bassant A. Elbaz a, I.M. Fares a, Ali M. Ahmed b, Ibrahim M. Hegab Egypt
8 Communications Biology volume 6, Article number: 370 (2023)  CRISPR-clear imaging of melanin-rich B16-derived solid tumors Rajib Schubert, Taegeun Bae, Branko Simic, Sheena N. Smith, Seong-Ho Park, Gabriela Nagy-Davidescu, Viviana Gradinaru, Andreas Plückthun & Junho K. Hur  USA
9 Aquaculture Reports
Volume 28, February 2023, 101434
Ammonia toxicity in Nile tilapia: Potential role of dietary baicalin on biochemical profile, antioxidant status and inflammatory gene expression Gehad E. Elshopakey a, Heba H. Mahboub b, Nagwa I. Sheraiba c, Maram H. Abduljabbar d, Yasmina K. Mahmoud e, Mosleh M. Abomughaid f, Ayman K. Ismail Egypt
10 MDPI 2023 Comparison of Immune Response of Litopenaeus vannamei Shrimp Naturally Infected with Vibrio Species, and after Being Fed with Florfenicol Medhat S. Shakweer 1,Gehad E. Elshopakey 2ORCID,Abdelwahab A. Abdelwarith 3ORCID,Elsayed M. Younis 3,Simon John Davies 4ORCID andSamia Elbahnaswy 1,* Egypt
11 Journal of Experimental & Clinical Cancer Research volume 42, Article number: 42 (2023) GPX8 regulates clear cell renal cell carcinoma tumorigenesis through promoting lipogenesis by NNMT Tin Tin Manh Nguyen, Thi Ha Nguyen, Han Sun Kim, Thien T. P. Dao, Yechan Moon, Munjun Seo, Sunmi Kang, Van-Hieu Mai, Yong Jin An, Cho-Rok Jung, Jin-Mo Kim & Sunghyouk Park  Vietnam

 

TroubleShooting Guide
QAfter transferring aqueous layer, can layer be separated after using binding buffer?
AAfter lysis, it is separated by centrifugation after chloroform treatment. After that, there is no layer separation phenomenon when the solution in the upper layer and the binding buffer are mixed. Therefore, all of the mixed solution should be loaded in the column.
QWhile using easy-spin kit, there is no enough lysis buffer. Can I purchase it separately?
Aeasy-spin Lysis Buffer is same as #17061 easy-BLUE.
QIs it possible to extract the RNA using the easy-spin kit and treat the isopropanol after storing it?
AIt is possible to store at refrigerator for one week. You do not need to add isopropanol because this kit is possible to store at refrigerator for one week.
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