easy-spin™ Total RNA Extraction Kit
Spin type product which can extract high purity and yield total RNA without any genomic DNA contamination easily and quickly from cells and tissues
1. High purity and high yield RNA extraction
easy-spin™ Total RNA Extraction Kit is designed to extract total RNA from cell and tissue in easy and fast way without mixing of genomic DNA. RNA extraction product is normally divided into column type and solution type. Solution type (phenol use) has higher yield comparing to spin type and also can extract large amount of RNA. Even though alcohol precipitation step is required due to the use of phenol, it has been widely used to extract RNA. On the contrary, spin type does not required alcohol precipitation to extract pure RNA, but it is mainly used for the rapid extraction of RNA from many samples, or for the safety and eco-friendliness of experimenters and the ease of experimentation.
As described above, the RNA extracted in the above two forms should have enough purity and yield to be used in almost all molecular biological experiments such as Northern blot analysis, cDNA synthesis and RT-PCR. It is a easy-spin™ (DNA free) Total RNA Extraction Kit that combines the advantages of the solution type and the column type. This product eliminates the inconvenience of the alcohol PPT stage when using the solution type product, and total RNA can be extracted within 30 minutes without contamination of genomic DNA. This product significantly reduces contamination concerns by using the CAPS method to individually pack the columns to effectively perform pathogen research (see Figure 1).
[ Figure 1. Spin Column & CAPS Information ]
2. Obtaining high-quality RNA suitable for RNA-Seq/NGS genome analysis
The purity of RNA is generally assessed using a spectrophotometer by measuring the A260/A280 ratio (nucleic acid to protein ratio, with 2.0 indicating pure RNA) and the A260/A230 ratio (nucleic acid to contaminants such as phenol, with 2.0-2.2 indicating pure RNA). However, purity and quality are distinct; even if the purity is high, the quality can be rated low if the RNA is degraded. The integrity of RNA is assessed using the RNA Integrity Number(RIN) measured by the Bioanalyzer from Agilent Technologies. The RIN value is provided on a scale from 1 to 10 based on the RNA band pattern, the 28S:18S (or 23S:16S) rRNA ratio, and background noise (degraded RNA fragments). For RNA-Seq, it is generally recommended for RNA to have a minimum RIN value of 7.0 or higher and a concentration of at least 1 μg. If RNA is degraded, sequencing accuracy can decrease, and gene expression levels may be distorted; therefore, high-concentration and high-quality RNA purification is essential. This product efficiently extracts high-quality RNA (RIN value 8.0 or higher) from various biological samples and is suitable for genome analysis research.
Contents | 50 COLUMNS |
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Lysis Buffer(easy-BLUE Solution) | 50ml × 1 ea |
Binding Buffer | 20ml × 1 ea |
Washing Buffer A | 40ml × 1 ea |
Washing Buffer B(Concentration) | 10ml × 1 ea |
Elution Buffer | 20ml × 1 ea |
Spin Columns(Orange color) | 50 Columns |
Collection Tubes | 50 tubes |
Manual | 1 ea |
Comparison of extraction efficiency of total RNA according to various product groups
Total RNA was isolated and purified from SNU-1 cells using the easy-spin™ Total RNA Extraction Kit and competitors products; solution type and column type. In the case of extraction using the easy-spin™ Total RNA Extraction Kit, we could see that the extracted total RNA was superior to the total RNA extracted by the competitors products.
Lane M; marker; lane 1, easy-spin™ Kit; lane 2, supplier A(phenol type); lane 3, supplier B(column type)
Results of genomic DNA contamination
We confirmed the presence of gDNA contamination by PCR amplification using IL-10 primer and total RNA extracted from easy-spin™ Total RNA Extraction Kit and competitors products; solution type and column type. In the case of the competitors products, gDNA contamination was confirmed in phenol type and column type, while we could confirm that gDNA contamination was negative in the RNA extracted by the easy-spin™ Total RNA Extraction Kit.
Lane M; marker; lane 1, easy-spin™ Kit; lane 2, supplier A(column type); lane 3, supplier B (phenol type); lane P, control template gDNA
RNA Extraction Performance for Various Samples (RIN Value)
1 | Annals of the Romanian Society for Cell Biology, 8092–8106 | The Potential Role of Vitamin D Supplementation in Ameliorating the Pathogenesis of Induced Type 2 Diabetes in Rats | Mona I. Abdel Ati, Heba F. Pasha, Amal M. El-Gayar, Mamdouh M. El-Shishtawy | Iraq |
2 | Wiley Online Library, 27 June 2022 https://doi.org/10.1111/are.15963 | Validation of reference genes for gene expression analysis in melanin-injected black tiger shrimp (Penaeus monodon) | Hong-Nhung T. Phan,Hong-Loan T. Nguyen,Ha-Giang Vu,Dinh-Thang Nguyen,Thai Nho Dinh,Lua T. Dang,Trang Thi Ngo,Le-Na T. Nguyen,Tuan-Nghia Phan | Vietnam |
3 | Life Sciences, Volume 297, 15 May 2022, 120443 | Antitumor effects of rhamnazinon sorafenib-treated human hepatocellular carcinoma cell lines via modulation of VEGF signaling and PI3K/NF-κB p38/caspase-3 axes cross talk | Yasmin H.HabibaaGamal A.OmranaMaged W.HelmybMaha E.Houssena | Egypt |
4 | Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1864, Issue 11, 1 November 2022, 184014 | Activation of serotonin receptor 2 by glucosylsphingosine can be enhanced by TRPA1 but not TRPV1: Implication of a novel glucosylsphingosine-mediated itch pathway | RamshaAfzalaWon-SikShimbc | Korea |
5 | Research
Square, March 22nd, 2022, DOI:
https://doi.org/10.21203/rs.3.rs-1420695/v1 |
Functional
Relationship of Interferon Regulator Factor-5, Interferon-γ, and Hypoxia-Induced Factor-1α with COVID-19 During Pregnancy Outcomes |
Dilsad
Herkiloglu, Omer Erdogan, Erkan Gumus, Ozge Cevik, Sarfraz Ahmad |
USA |
6 | Springer, Medical Oncology volume 39, Article number: 143 (2022) | Unraveling the therapeutic potential of GANT61/Dactolisib combination as a novel prostate cancer modality | Mohamed Youssef, Nermine Moussa, Maged W. Helmy & Medhat Haroun | Egypt |
7 | Applied
Animal Behaviour Science Volume 261, April 2023, 105902 |
Different LED light colors modify behavior, physiology, and hypothalamic CRF and NPY mRNA expression in Japanese quail (Coturnix coturnix Japonica) | Bassant A. Elbaz a, I.M. Fares a, Ali M. Ahmed b, Ibrahim M. Hegab | Egypt |
8 | Communications Biology volume 6, Article number: 370 (2023) | CRISPR-clear imaging of melanin-rich B16-derived solid tumors | Rajib Schubert, Taegeun Bae, Branko Simic, Sheena N. Smith, Seong-Ho Park, Gabriela Nagy-Davidescu, Viviana Gradinaru, Andreas Plückthun & Junho K. Hur | USA |
9 | Aquaculture
Reports Volume 28, February 2023, 101434 |
Ammonia toxicity in Nile tilapia: Potential role of dietary baicalin on biochemical profile, antioxidant status and inflammatory gene expression | Gehad E. Elshopakey a, Heba H. Mahboub b, Nagwa I. Sheraiba c, Maram H. Abduljabbar d, Yasmina K. Mahmoud e, Mosleh M. Abomughaid f, Ayman K. Ismail | Egypt |
10 | MDPI 2023 | Comparison of Immune Response of Litopenaeus vannamei Shrimp Naturally Infected with Vibrio Species, and after Being Fed with Florfenicol | Medhat S. Shakweer 1,Gehad E. Elshopakey 2ORCID,Abdelwahab A. Abdelwarith 3ORCID,Elsayed M. Younis 3,Simon John Davies 4ORCID andSamia Elbahnaswy 1,* | Egypt |
11 | Journal of Experimental & Clinical Cancer Research volume 42, Article number: 42 (2023) | GPX8 regulates clear cell renal cell carcinoma tumorigenesis through promoting lipogenesis by NNMT | Tin Tin Manh Nguyen, Thi Ha Nguyen, Han Sun Kim, Thien T. P. Dao, Yechan Moon, Munjun Seo, Sunmi Kang, Van-Hieu Mai, Yong Jin An, Cho-Rok Jung, Jin-Mo Kim & Sunghyouk Park | Vietnam |
Product | Cat.No | Capacity | inquire | ||||
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easy-BLUE™ Total RNA Extraction Kit Best |
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Maxime™ RT-PCR PreMix Best |
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Power cDNA Synthesis Kit |
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