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RNA-spin™ Total RNA Extraction Kit

Cat.No Capacity Inquire
17211 50 col. Inquire
PRODUCT INFORMATION
Description

Spin type product that can extract total RNA quickly and easily from cells and tissues

  • • Extraction of high yield and purity total RNA within 20 minutes from cells and tissues
  • • Unnecessary of organic solvent or alcohol PPT procedure
  • • High reproducible results
  • • Accurate downstream applications available by minimization of cross-contaminant
  • • Minimization of contamination concerns through application of CAPS method

RNA-spin™ Total RNA Extraction Kit is a spin-type product designed to extract total RNA quickly and easily from cells or tissues. Unlike the easy-spin™ (DNA free) Total RNA Extraction Kit, it uses chaotropic salt and detergent to effectively dissolve cells or tissues, denature nucleic acid proteins and inhibit RNase activity derived from cells without using phenol. In other words, R-Buffer and beta-mecaptoethnol in RNA-spin™ Total RNA Extraction Kit effectively inactivate RNase and denature cells and tissue proteins, so it allows RNA to be freely dissolved from proteins.

In addition to this, it is possible to effectively attach RNA only to the silica-gel membrane together with EtOH added after the dissolution process, thereby it helps the contamination of DNA and protein to be minimized. As mentioned above, this product can be safely used because it does not use harmful organic solvent, even it can extract only pure RNA very quickly and easily without EtOH precipitation. Total RNA extracted by RNA-spin™ Total RNA Extraction Kit has enough high purity and yield to be used for cDNA synthesis, RT-PCR, Northern Blot analysis, Dot Blot and primer extension etc. As the columns are packed individually by CAPS, contamination is remarkably reduced (see Figure 1).

[ Figure 1. Spin Column & CAPS Information ]

Applications
  • 01Northern blot analysis
  • 02cDNA synthe
  • 03RT-PCR, Real-time RT-PCR
  • 04Dot Blotting
  • 05Primer extension
  • 06Infectious disease research, etc.
Kit Contents
Contents 50 COLUMNS
R-Buffer 20ml × 1 ea
Washing Buffer A 40ml × 1 ea
Washing Buffer B(Concentration) 10ml × 1 ea
Elution Buffer 20ml × 1 ea
Spin Columns(Orange color) 50 Columns
Collection tubes 50 tubes
Manual 1 ea
Technical Data

RNA extraction efficiency from animal cultured cells and tissues

The RNA-spin™ Total RNA Extraction Kit is suitable for RNA extraction from animal cultured cells and tissues.

lane 1, SNU 601(Human); lane 2, B16(Mouse); lane 3, Spleen (Mouse); lane 4, Kidney(Mouse)

Comparison test with competitor product

In cell and tissue samples, the RNA-spin™ Total RNA Extraction Kit provided superior extraction efficiency compared to competitor product. (As competitive product, single column type product of non-phenol type was used.)

TroubleShooting Guide

 

QWhat is the differences between easy-spin kit and RNA-spin kit?
Aeasy-spin kit is made by combining column type and solution type and RNA-spin kit is made by only column type.
QIs it possible to prepare RNA Prep using RNA-spin column after taking out aqueous layer with Trizol?
AWe sell easy-spin kit for RNA extraction. easy-spin kit has designed to customer taste. The lysis step is the same as the instruction, and RNA can be extracted with high purity and high yield using a column when RNA is recovered. Since the RNA-spin kit does not use an organic solvent, the recovery rate can not be guaranteed for column loading after lysis with Trizol. Therefore, we recommend using easy-spin, which has advantages of solution type and advantages of column type.
QRNA was extracted using RNA-spin and we used elution buffer provided from kit. Extracted RNA is use with metal but metal can change because of chemical and this led bad test results. If it possible, can I know components for elution buffer?
ABased on our regulation, it is impossible to tell you the components. In case of using elution buffer, you can replace DEPC water for RNase free water. Please use RNase free water instead of using elution buffer.

 

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