Life Science (Reagent)We will share various news about iNtRON


Viral Gene-spin™ Viral DNA/RNA Extraction Kit

Cat.No Capacity Inquire
17151 50 col. Inquire

Spin type product that can extract viral DNA/RNA easily and rapidly from the various clinical samples such as serum, plasma, cell free body fluid and stool etc.

  • • Extraction of Viral DNA/RNA from various clinical samples within 20 minutes
  • • High yield and purity viral DNA / RNA extraction with powerful lysis buffer
  • • Easy extraction without enzyme treatment
  • • Be used safely with non-phenol method and no need for EtOH precipitation
  • • OIE listed product based on various clinical sample results
  • • Be useful for molecular diagnostic studies on clinical samples by minimizing contamination by CAPS

Viral Gene-spin™ Viral DNA/RNA Extraction Kit is designed to extract virus DNA/RNA easily and quickly from the various clinical samples such as serum, plasma, cell-free fluid, stool, whole blood, tissue, swab, urine, cultured cell and virus infection tissue, etc. Viral Gene-spin™ Viral DNA/RNA Extraction Kit has a very powerful lysis buffer that effectively dissolves and denatures virus envelope and capside protein without additional enzyme treatment (except some viruses) to easily elute viral gene and effectively inactivate RNase. The binding buffer added after the elution helps to attach genes only to silica-gel membrane, and two different washing buffers efficiently remove proteins and other contaminants to get high purity viral gene.

The Viral Gene-spin™ Viral DNA/RNA Extraction Kit does not use harmful organic solvents, so it can be used safely. Even it can extract pure viral gene very quickly without EtOH precipitation. In addition, CAPS is applied to columns to minimize the risk of contamination (see Figure 1). The viral genes isolated and purified from the Viral Gene-spin™ Kit can be used for viral diagnostics, cancer research etc. Especially, it is useful for clinical diagnosis of people or animals that have been infected by virus or bacteria.

Figure 1. Spin Column & CAPS Information

  • 01Pathogen detection
  • 02Microarray
  • 03cDNA synthesis
  • 04Reverse Transcriptase PCR(RT-PCR)
  • 05Quantitative PCR(qPCR, qRT-PCR) etc.
Kit Contents
Contents 50 COLUMNS
Lysis Buffer 30ml × 1 ea
Binding Buffer 40ml × 1 ea
Washing Buffer A 30ml × 1 ea
Washing Buffer B(Concentration) 10ml × 1 ea
Elution Buffer 20ml × 1 ea
Spin Columns(Orange color) 50 Columns
Collection Tubes 50 tubes
Manual 1 ea
Technical Data

Sensitivity test of HBV (Human hepatitis B virus, DNA virus)

HBV DNA was extracted from sera of HBV-infected patients using the Viral Gene-spin™ Viral DNA/RNA Extraction Kit. As a result of sensitivity test, PCR inhibitions were less because cross-contaminants were completely removed.

Detection limit test of Enterovirus (RNA virus) by Real-time RT-PCR

Viral Gene-spin™ Viral DNA/RNA Extraction Kit was used to extract EV RNA from stool suspension of EV-infected patients, then Real-time RT-PCR was performed. As the result, we could see that viral RNA was properly isolated by the Viral Gene-spin™ Viral DNA/RNA Extraction Kit.



Citation List 


1 Tropical Animal Health and Production
January 2014, Volume 46, Issue 1,  pp 271–277
Molecular epidemiology of Newcastle disease viruses in Vietnam Kang-Seuk Choi Email author , Soo-Jeong Kye, Ji-Ye Kim, Thanh Long To, Dang Tho Nguyen, Youn-Jeong Lee, Jun-Gu Choi, Hyun-Mi Kang, Kwang-Il Kim, Byung-Min Song, Hee-Soo Lee
2 2014 Summer; 5(3): 187–191.  Phylogenetic characterization of the fusion genes of the Newcastle disease viruses isolated in Fars province poultry farms during 2009-2011 Mohammad Javad Mehrabanpour,1,* Setareh Khoobyar,2 Abdollah Rahimian,1 Mohammad Bagher Nazari,3 and Mohammad Reza Keshtkar4
3 November 2014, Volume 98, Number 11
Page 1590
First Report of Chickpea chlorotic dwarf virus Infecting Hot Pepper in India H.-S. Byun, E.-J. Kil, S. Kim, and H. Hwang, Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea; J. H. Lee, Nongwoo Bio, Suwon, Korea; Y.-J. Chung,
4  8 January 2014, doi: 10.1128/CVI.00636-13   Clin Vaccine Immunol March 2014   vol. 21  no. 3  360-365  Virus-Like Particle Vaccine Confers Protection against a Lethal Newcastle Disease Virus Challenge in Chickens and Allows a Strategy of Differentiating Infected from Vaccinated Animals  Jae-Keun Parka, Dong-Hun Leea, Seong-Su Yuka, Erdene-Ochir Tseren-Ochira, Jung-Hoon Kwona, Jin-Yong Noha, Byoung-Yoon Kima,  Soo-Won Choia,  Sang-Moo Kangb,  Joong-Bok Leea,  Seung-Yong Parka,In-Soo Choia and Chang-Seon Songa
5 Journal
 Avian Pathology  
Volume 44, 2015 - Issue 1 
Genetic characterization of three novel chicken parvovirus strains based on analysis of their coding sequences Bon-Sang Koo, Hae-Rim Lee, Eun-Ok Jeon, Moo-Sung Han, Kyeong-Cheol Min, Seung-Baek Lee
6 J Bacteriol Virol. 2015 Dec;45(4):319-327. English. Biological Property of Recombinant Hemagglutinin-Neuraminidase Protein of Avian Paramyxovirus Type 6 Expressed by Recombinant Baculovirus Ji-Ye Kim,1 Hyun-Jeong Lee,1 Soo-Jeong Kye,2 Saeromi Kim,1 Hee-Jung Seul,1 Sang-Eun Kim,4 Hee-Soo Lee,3 Suk-Chan Jung,1 and Kang-Seuk Choi
Korean J Intern Med. 2015 Jan; 30(1): 96–103.
Utilization of the respiratory virus multiplex reverse transcription-polymerase chain reaction test for adult patients at a Korean tertiary care center Mi Young Ahn,1 Seong-Ho Choi,corresponding author1 Jin-Won Chung,1 and Hye Ryoun Kim2
8 Plant Pathology, Volume64, Issue6
December 2015
Pages 1284-1291
Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)  J. Kim , E.J. Kil , S. Kim , H. Seo, H.S. Byun , J. Park, M.N. Chung , H.R. Kwak,
9 J. Microbiol. Biotechnol. (2016), 26(12), 2206–2213
Identification of Uncommon Candida Species Using Commercial
Identification Systems
Tae-Hyoung Kim1, Oh Joo Kweon2, Hye Ryoun Kim2, and Mi-Kyung Lee
10 Future Virology, Vol. 11, No. 3 | , Published Online:22 Feb 2016 Human metapneumovirus frequency in Iranian children with respiratory symptoms A Sanaei Dashti, SMH Emamifar, T Hashempour
11 Cluster randomised controlled trial to examine medical mask use as source control for people with respiratory illness Chandini Raina MacIntyre1,2, Yi Zhang3, Abrar Ahmad Chughtai1,2, Holly Seale1,2, Daitao Zhang3, Yanhui Chu3, Haiyan Zhang3, Bayzidur Rahman1,2, Quanyi Wang3
12 Scientific Reports volume 6, Article number: 19013 (2016)
Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes Eui-Joon Kil, Sunhoo Kim, Ye-Ji Lee, Hee-Seong Byun, Jungho Park, Haneul Seo, Chang-Seok Kim, Jae-Kyoung Shim, Jung-Hwan Lee, Ji-Kwang Kim, Kyeong-Yeoll Lee, Hong-Soo Choi & Sukchan Lee
13 19 Jan 2016 First Isolation of Severe Fever with Thrombocytopenia Syndrome Virus from Haemaphysalis longicornis Ticks Collected in Severe Fever with Thrombocytopenia Syndrome Outbreak Areas in the Republic of Korea Roh Jong Yul , Lee Ye-Ji , Park Won Il , Han Myung Guk , Ju Young Ran and Lee Won-Ja
14 Vector-Borne and Zoonotic DiseasesVol. 16, No. 1 First Isolation of Severe Fever with Thrombocytopenia Syndrome Virus from Haemaphysalis longicornis Ticks Collected in Severe Fever with Thrombocytopenia Syndrome Outbreak Areas in the Republic of Korea Yun Seok-Min , Song Bong Gu , Choi WooYoung , Roh Jong Yul , Lee Ye-Ji , Park Won Il , Han Myung Guk , Ju Young Ran , and Lee Won-Ja
15 September 2016, Volume 100, Number 9
Page 1958
First Report of Papaya leaf curl virus in Papayas in Korea and Recovery of its Symptoms H.-S. Byun, E.-J. Kil, and H. Seo,J.-K. Kim
16 J Vet Sci. 2016 Sep;17(3):323-330. English.
Published online September 20, 2016. 
The current epidemiological status of infectious coryza and efficacy of PoulShot Coryza in specific pathogen-free chickens
Moo-Sung Han,1 Jong-Nyeo Kim,1 Eun-Ok Jeon,1 Hae-Rim Lee,1 Bon-Sang Koo,1 Kyeong-Cheol Min,1 Seung-Baek Lee,1 Yeon-Ji Bae,1 Jong-Suk Mo,1 Sun-Hyung Cho,1 Hye-Sun Jang,2 and In-Pil Mo
17 J Vet Sci. 2017 Mar;18(1):89-94. English. Phylogeographical characterization of H5N8 viruses isolated from poultry and wild birds during 2014–2016 in South Korea Byung-Min Song, Eun-Kyoung Lee, Yu-Na Lee, Gyeong-Beom Heo, Hee-Soo Lee and Youn-Jeong Lee
18 Journal of Wildlife Diseases 53(4):749-760. 2017  MOLECULAR EPIDEMIOLOGY OF AVIAN POXVIRUS IN THE ORIENTAL TURTLE DOVE (STREPTOPELIA ORIENTALIS) AND THE BITING MIDGE (CULICOIDES ARAKAWAE) IN THE REPUBLIC OF KOREA Hae Rim Lee, Bon-Sang Koo, Jong-Taek Kim, Heung-Chul Kim, Myung-Soon Kim, Terry A. Klein, Man-Seok Shin, Sanghun Lee, Eun-Ok Jeon, Kyung-Cheol Min, Seung Baek Lee, Yeonji Bae 
19 Veterinary Microbiology

Volume 207, August 2017, Pages 178-180
Prevalence of novel porcine circovirus 3 in Korean pig populations Taeyong Kwon a, Sung J. Yoo a, Choi-Kyu Park b, Young S. Lyoo a
20 Veterinary Microbiology

Volume 199, February 2017, Pages 54-61
Genetic diversity of ORF 4–6 of type 1 porcine reproductive and respiratory syndrome virus in naturally infected pigs Dong-Uk Lee a, Sung J. Yoo a, Taeyong Kwon a, Sang H. Je a, Jeong Y. Shin a, Jeong J. Byun a, Myung H. Kim b, Young S. Lyoo a
21 Molecular Pathology in Clinical Practice pp 755-778 Respiratory Infections Christine C. Ginocchio
22 Aquaculture Reports
Volume 7, August 2017, Pages 57-65
open access
The presence of Vibrionaceae, Betanodavirus and Iridovirus in marine cage-cultured fish: Role of fish size, water physicochemical parameters and relationships among the pathogens AzilaAbdullahaRimatulhanaRamliaMohd Syafiq MohammadRidzuanaMuniraMurniaShahidanHashimaFahmiSudirwanaSiti ZahrahAbdullahaNur NazifahMansorbSofeaAmirabMohd ZamriSaadceMohammad Noor AzmaiAmal
23 Vol 7, No 2 (2018) > Kaleibar  Prevalence of Bovine Immunodeficiency Virus Infection in Buffaloes in East Azerbaijan, Northwestern Iran Mohammad Tolouei- Kaleibar, Morteza Mozaffari, Javad Ashrafi, Golamreza Nikbakht, Ezzatollah Fathi
24 Viruses 2018, 10(2), 78; doi:10.3390/v10020078  First Diagnosed Case of Camelpox Virus in Israel Oran Erster 1,†, Sharon Melamed 2,†, Nir Paran 2, Shay Weiss 2, Yevgeny Khinich 1, Boris Gelman 1, Aharon Solomony 3 and Orly Laskar-Levy 
25 Received: 28 March 2018 | Revised: 29 May 2018 | Accepted: 31 May 2018 DOI: 10.1111/jfd.12843
] First detection of tilapia lake virus (TiLV) in wild river carp (Barbonymus schwanenfeldii) at Timah Tasoh Lake, Malaysia Azila Abdullah1 | Rimatulhana Ramly1 | Mohammad Syafiq Mohammad Ridzwan1 | Fahmi Sudirwan1 | Adnan Abas2 | Kamisa Ahmad1 | Munira Murni1 | Beng Chu Kua1
26 Journal of Clinical pathology, Volume 71, Issue 4 Increased incidence of BK virus viraemia among patients undergoing chronic haemodialysis: a case–control study  Samer Fuad Swedan
27 ASIAN J TROP BIOTECHNOL ISSN: 0216-6887 Volume 18, Number 1, June 2021 E-ISSN: 2301-8658 Pages: 13-27  Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples  ALFI SOPHIAN♥, RATNA PURWANINGSIH, MUINDAR, EKA PUTRI JUNIARTI IGIRISA, MUHAMMAD LUTHFI AMIRULLAH 
28 APS Publications First Report of Fig Mosaic Virus on Fig in Russia S. Chirkov, S. Tsygankova, S. Rastorguev, I. Mitrofanova, S. Chelombit, E. Boulygina, N. Slobodova, and F. Sharko
29 APS Publications First Report of Duranta leaf curl virus Infecting Ficus virens Showing Leaf Curl Symptoms in Pakistan Aamir Lal, Eui-Joon Kil, Thuy Thi Bich Vo, Chairina Fadhila, Phuong Thi Ho, Malik Nawaz Shuja, Muhammad Ali, and Sukchan Lee
30 Wiley Online Library Molecular characterization of infectious bronchitis virus based on RNA-dependent RNA polymerase gene Mozafar Hajijafari Anaraki,Nariman Sheikhi,Hadi Haghbin Nazarpak,Gholamreza Nikbakht Brujeni
31 Iran J Vet Res. 2021 Spring; 22(2): 155–160. Molecular characterization and phylogenetic analysis of VIIl sub-genotype of avian orthoavulavirus 1 M. Khosravi,1,* S. Seifi,2 and Z. Tazeh3
32 Al-Qadisiyah journal of pure science The effect of age ,gender, type of feeding and receiving atreatment on the distribution of Enterovirus infection among chidren Mohammed Sarim Al-fatlawi, Ghaidaa J. Mohammed
33 Research, 10 Jan 2023 An Effective Integrated Machine Learning Framework for Identifying Severity of Tomato Yellow Leaf Curl Virus and Their Experimental Validation NATTANONG BUPI, VINOTH KUMAR SANGARAJU, LE THI PHAN, AAMIR LAL, THUY THI BICH VO, PHUONG THI HO, MUHAMMAD AMIR QURESHI, MARJIA TABASSUM, SUKCHAN LEE , AND BALACHANDRAN MANAVALAN


TroubleShooting Guide
QAfter infecting virus gene with cell line, experiment for checking cell condition has performed. Is it possible to only extract virus gene in order to use it for sequencing.
AThis kit not only extract virus but also genome(DNA/RNA). Therefore, it is recommended to sequester the target virus gene by amplifying and purifying the target virus gene by PCR.
QI am willing to extract only virus DNA, can lysis buffer lysed host’s nucleus membrane?
AThe nucleus membrane can also be lysed with the lysis buffer in the kit. Therefore, not only the virus gene but also the genome (gDNA, RNA) of the host cell can be extracted. This extracted DNA can be used as a sample for the detection of virus using PCR but it is impossible to isolate the nucleic acid of pure virus. The confirmation of virus infection can be determined by PCR or RT-PCR.
QI want to confirm infection of PCV from Pig’s tissue. Is it possible to extract viral DNA from tissue? If this works, please provide me protocol.
AThis kit is possible to extract viral gene from tissue. Generally, we need to prepare 10 to 25mg of sample, but we recommend 50mg for heart, brain muscle tissues. The protocol recommends to homogenize the Tissue and add PBS to make a total volume of 150ul. Then, move onto next step.
QWe are currently developing a type B infection treatment. We are planning to extract HBV from HBV infected blood.
AWe recommend Viral-gene spin Viral DNA/RNA Extraction kit. Viral-gene spin product has designed to easily extract viral genes from samples of various liquid components such as serum, plasma and cell culture within 20 minutes.
QWhen we apply the HBV infected blood sample for PCR using designed primer, however, no bands are shown while performing amplification. What is the solution?
AThis product is a product that can extract DNA or RNA virus from various liquids such as serum, plasma, etc., various liquid such as tissue emulsion liquid, feces suspension liquid, and cell culture liquid. You should first check the purity and yield of the extracted HBV sample and design the appropriate PCR condition accordingly. I think it is better to run PCR after confirming the specificity and sensitivity of the designed primer.
Related Product
관련제품 Product,No,Capacity,Inquire list
Product Cat.No Capacity inquire
i-Taq™ DNA Polymerase
25021 250 unit Inquire
25022 500 unit Inquire
i-StarTaq™ DNA Polymerase
25161 250 unit Inquire
25162 500 unit Inquire
Maxime™ PCR PreMix (i-Taq)
25025 96 tubes (20 ㎕) Inquire
25026 96 tubes x 5 ea Inquire
Maxime™ PCR PreMix (i-StarTaq)
25165 96 tubes (20 ㎕) Inquire
25167 96 tubes x 5 ea Inquire
Maxime™ PCR PreMix (i-StarMAXⅡ)
25281 96 tubes Inquire
SiZer™-100 DNA Marker Solution
24073 0.5 ㎖ Inquire
SiZer™-1,000 DNA Marker Solution
24074 0.5 ㎖ Inquire
RedSafe™ Nucleic Acid Staining Solution 
21141 1 ㎖ Inquire