i-StarTaq™ DNA Polymerase
High-specificity i-StarTaq ™ PCR core Kit with hot-start to reduce non-specific amplification
i-StarTaq ™ DNA Polymerase is a non-antibody, chemical-based, hot-start enzyme that reduces nonspecific amplification and improves specificity and accuracy. Therefore, it can be used as a solution when the specific band is weakly amplified by nonspecific amplification such as nonspecific band or primer dimer.Although Taq DNA Polymerase is most commonly used in PCR, as the number of targets to be amplified varies, Taq DNA Ppolymerase is not suitable for some PCR conditions. The most frequently encountered problem in PCR is that the non-specific band is amplified together or the amplification of the specific band is remarkably low or fails, mainly due to the low specificity. This happens because either the forward or reverse primer is less specific or the two primers are more prone to primer dimers because Taq DNA Polymerase is usually active at room temperature to prepare the PCR reaction solution. Hot-start PCR is a method designed to overcome this problem by blocking the physicochemical reaction of Taq DNA Polymerase to prevent it from working below the annealing temperature of the primer. Classically, we used a physical method to start the reaction by adding Taq DNA Polymerase to the PCR reaction solution heated to 94 ℃ or to physically isolate the reaction solution and Taq DNA Polymerase using wax. Currently, we perform hot-start PCR using a specific antibody or chemically inactivating Taq DNA Polymerase.As described above, i-StarTaq ™ DNA Polymerase is a hot-start PCR method which is a chemical method and is suitable for general PCR and RT-PCR as well as Multiplex PCR and disease diagnosis research.
|Content||250 Units||500 Units|
|i-StarTaq™ DNA Polymerase(5U/ml)||250 units x 1 tube||500 units x 1 tube|
|10× PCR Buffer(w/20 mM Mg2+)||1 ml x 1 tube||1 ml x 1 tube|
|10× Mg2+ free PCR Buffer||1 ml x 1 tube||1 ml x 1 tube|
|10 mM dNTPs (2.5 mM/each)||500 μl x 1 tube||1 ml x 1 tube|
|25 mM Mg2+||1 ml x 1 tube||1 ml x 1 tube|
|Manual||1 ea||1 ea|
Optimal amounts of templates from different origins
Sensitivity comparison data from Multiplex PCR
Using the i-StarTaq ™ DNA Polymerase and Hot-start DNA Polymerase from Company A and B, the sensitivities of the multiplex primers (fyuA (780 bp), tsh (420 bp), and Irp2 (280 bp) As can be seen from the results, i-StarTaq ™ DNA Polymerase shows excellent sensitivity at low template concentrations.
Lane M, 100 bp DNA Marker; lane 1, Negative control; lane 2, 100 ng gDNA, Lane 3, 50 ng gDNA; lane 4, 25 ng gDNA; lane 5, 12.5 ng gDNA; la ne 6, 6.25 ng gDNA; lane 7, 3.125 ng gDNA; lane 8, 1.5625 ng gDNA