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DNA-maxi™ SV Plasmid DNA Purification Kit

Cat.No Capacity Inquire
17253 12 col. Inquire
PRODUCT INFORMATION
Description

Spin & Vacuum type for separating/ purifying high yield of Plasmid DNA (Maxi Scale)

  • • Possible to separate and purify plasmid DNA effectively (Maxi Scale)
  • • High copy number : 100~150 ml culture volume
  • • Low copy number : 300~400 ml culture volume
  • • Possible to separate and purify high yield and purified plasmid DNA within 40minutes (Vacuum type)
  • • Possible to remove Cell lysate easily using Pre-filtration column
  • • Ethanol precipitation is not necessary by using “Swing bucket rotor only” method
  • • Separation/purification of plasmid DNA over 30kb

DNA-maxi™ SV Plasmid DNA Purification Kit is used for purifying and separating high yield of plasmid DNA from maxi scale of bacterial cell culture volume using silica membrane and optimized alkali lysis buffer system. It is composed of two column system including binding column(pre-filtration column and DNA binding membrane) in order to remove cell lysate easily and to perform separation/purification using vacuum method. (Figure 1.reference. Pre & Binding Column Combination)Neutralization buffering system used for pH neutralization and binding can perform not only separation/purification for plasmid DNA over 30Kb, but also remove chromosomal DNA, cell debris effectively after lysis. Also, pH change through alkaline lysis method can be observed visually in real time because the product contains lysis & neutralization indicator such as Lysis Viewer. Product is used for either High-copy plasmid DNA or low-copy plasmid DNA and in order to perform separation and purification effectively from endA+ strain(HB101, the JM strains, NM series strains, PR series strains, etc.), Washing Buffer A is provided separately.

Applications
  • 01Ligation & transformation
  • 02PCR & Real-time PCR
  • 03Automated fluorescent
    sequencing
  • 04Transfection
  • 05Restriction digestion etc.
Kit Contents
Contents 12 COLUMNS
M1 Buffer(Resuspension Buffer) 80ml × 1 ea
M2 Buffer(Lysis Buffer) 80ml × 1 ea
M3 Buffer(Neutralization Buffer) 80ml × 1 ea
Washing Buffer A 250ml × 1 ea
Washing Buffer B 30ml × 2 ea
Elution Buffer 30ml × 1 ea
Pre Column(Clear color) 12 Columns
Binding Column(Yellow color) 12 Columns
RNase A, lyophilized 60 mg x 1 tube
Manual 1 ea
Technical Data

Data Comparison for extraction efficiency based on size of plasmid DNA

After extracting various size of plasmid DNA using DNA-maxiTM SV plasmid DNA Purification Kit, the results verified plasmid DNA over 40kb is extracted.

Data Comparison for extraction efficiency based on various Host Strain

After extracting pUC 18 from each different type of host strain using DNA-maxiTM SV plasmid DNA Purification Kit, the results verified that different type of host strain does not affected test result.

TroubleShooting Guide
QWhat is culture volume and DNA recovery rate and purity?
AIt is specialized for Bacteria plasmid prep and prepare cultured sample for 12 to 16 hours at 37℃ and OD 600 should be 1.0~1.5. cultured solution for high copay is 100~150ml and Low copy is 300~400ml. DNA purity and recovery rate is depends on DNA size and strain.
QWhat does 'SV' mean?
AIt is abbreviation for 'column type and spin and vaccum type. When Spin protocol is used, please use swing type rotor. Because angle rotor is fixed by angle, so column elution or washing processes does not work well and it may cause low yield. Therefore please use swing rotor.
QHow long does it take to extract DNA?
ASpin and Vaccum types of protocol can be used. if spin type of protocol is used, it takes 80 to 90 minutes, and if vaccum type of protocol is used, it takes about 30 minutes.
QWhile performing experiment, I throw away collection tube by mistake, is it possible to replace with 50ml disposable tube?
AColumn is adjusted for 50ml disposable tube. Also, elution tube is not provided and elution tube is same as 50ml disposable tube. Therefore, it is able to use 50ml disposable tube.
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