2x PCR Master mix Solution (i-Taq™) is made from iNtRON’s i-Taq™ DNA Polymerase. i-Taq™ DNA Polymerase included in the product is the 94KDa thermostable DNA polymerase which is expressed in E.coli after cloning the polymerase gene of Thermus aquaticus (strain YT1). This removes protein originated from E.coli and DNA which can affect on PCR as contaminants to perform stable and effective DNA amplification and amplifies to maximum 5 Kb with genomic DNA or cDNA, etc. For the best polymerase activity regardless of the sort of template or the condition of reaction, buffer composition is optimized for the high reproducibility result.

2x PCR Master mix Solution (i-Taq™) is the product what is mixed every component: i-Taq™ DNA Polymerase, dNTP mixture, reaction buffer, and so on- in one tube. This is the product that can get the best result with the most convenience system. The first reason is that it has every components for PCR, so we can do PCR just add a template DNA, primer set, and D.W. The second reason is that it has Gel loading buffer to do electrophoresis, so we can do gel loading without any treatment. In addition, each batches are checked by a thorough Q.C., so its reappearance is high. It is suitable for various sample's experience by fast and simple using method. ​

Comparison with 2x PCR Master mix Solution (i-Taq™) and different company solution by amplifying 1Kb DNA fragment from variable amounts of λDNA Aliquots of 5 ㎕ in 20 ㎕ reaction are loaded on 1% agarose gel.

 

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 Citation List

 

1	The Egyptian Journal of Forensic Sciences and Applied Toxicology 2015 Vol.15 Issue 1, pp.1-15	Association of Genetic Polymorphisms of Paraxonase 1 ( Pon1 ) and Glutathione Stransferase ( M1 and T1 ) with Miscarriage in Pesticides Exposed Women at Zagazig University Hospitals	El-Fakharany , Yara M.  |  Arafa , Manar H.  |  Saraya , Yasser S.	Egypt
2	The Egyptian Journal of Aquatic Research Volume 46, Issue 3, September 2020, Pages 303-309	Occurrence of Penaeus aztecus, Ives, 1891 (Crustacea: Decapoda: Penaeidae) in the coastal water of Alexandria, Egypt	Rabab S.El-DeebaMoustafaSarhanbAmal R.KhafageaFatma A.Abdel RazekaMohammedAbdel-WahabbHamdy A.Omara	Egypt
3	Biodiversitas Journal of Biological Diversity	DNA barcoding of lamp shells (Brachiopoda: Lingula anatina) from Probolinggo, East Java, Indonesia	RENI AMBARWATI, DWI A. RAHAYU, FIDA RACHMADIARTI, FIRAS KHALEYLA	Indonesia
4	Research J. Pharm. and Tech. 2021; 14(6):3299-3306.	Cloning of Acetoacetyl-CoA reductase and Polyhydroxybutyrate synthase genes from the local isolate Bacillus aryabhattai 6N-NRC into E.coli	Neveen M. El-Metwally1*, Abd El-Nasser A	Egypt
5	Annals of the Romanian Society for Cell Biology, 7853–7865	Molecular Description of Aspergillus Species for Allergies and Asthma	Kenan Adnan Abdullah, Milad A. Mezher	Iraq
6	All Life, Volume 13, 2020 - Issue 1	The synergistic effect of fenretinide and metformin to achieve a decrease in insulin resistance and inflammatory mediators: an in vivo study	Mohamed M.A. Husseina Department	Egypt
7	IOP Conference Series: Earth and Environmental Science	Microscopical and molecular diagnosis of gastrointestinal nematodes infecting small ruminants in Menofia governorat	Ahmed Elkhatam1, Mahmoud R. AbouLaila2, Nasr Elbahy1 and Tamer M. Roshdy3	Malaysia
8	Veterinary Integrative Sciences, (2022). 20(1), 231–245.	Molecular detection of Chlamydia spp. and risk factors in farmed siamese crocodile in the mid-northeastern provincial cluster of Thailand	Sajana Thapa, Anucha Sirimalaisuwan, Kannika Na Lampang, Veerasak Panyapornwittaya, Warangkhana Chaisowwong	Thailand