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PCR

2X PCR Master mix Solution (i-Taq)

Cat.No Capacity Inquire
25027 0.5 ㎖ x 2 vials Inquire
PRODUCT INFORMATION
Description

2x PCR Master mix type product with high purity i-TaqTM DNA Polymerase that shows stable and efficient DNA amplification regardless of template type and reaction conditions 

  • • Ready to Use
       - All reagents required for the reaction are in solution in the tube
       - Immediate PCR by adding template and primer
  • • PCR reaction volume control
  • • 94 KDa thermostable DNA polymerase
  • • High purity Taq DNA Polymerase 
       - Removal of E. coli -derived proteins and DNA that may act as PCR sources 
  • • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • • Obtain high reproducible results 
  • • Applicable to DNA from cloned DNA to human genomic DNA

2x PCR Master Mix Solution (i-Taq ™) is designed to achieve the best results with a 2x master mix type in one tube, including all components required for PCR reaction, including i-Taq ™ DNA Polymerase . This product is able to perform PCR by adding template DNA, primer set and D.W only, and gel loading buffer is included, so gel loading can be done without any other treatment. The i-Taq ™ DNA Polymerase used in this product is a thermostable DNA polymerase of 94 kDa, which is expressed in E.coli by cloning the polymerase gene of Thermus aquaticus (strain YT1). The i-Taq ™ DNA Polymerase is an E. coli-derived protein It is purified to high purity by removing DNA. Stable and efficient DNA amplification is possible and it is possible to amplify up to 5 Kb from genomic DNA and cDNA. In addition, the 2x PCR Master Mix Solution (i-Taq ™) developed using this enzyme is a product that can be obtained with high reproducibility by optimizing the buffer composition to exhibit the best polymerase activity regardless of the template type or reaction conditions. It is not only convenient, it is highly reproducible, and it is a product that can be tested quickly and easily for various samples.

Applications
  • 01Genomic DNA, cDNA amplify 
  • 02T/A Cloning or Blunt-end Cloning

 

Kit Contents
Content 25027
2x PCR Master mix Solution(i-TaqTM) 0.5 ml x 2 tubes

 

 

Technical Data

Sensitivity Comparison data

In order to compare the sensitivity of 2x PCR Master Mix Solution (i-TaqTM) with that of other products, K562 genomic DNA was diluted by ½ concentration and subjected to IL-10 (1.3 Kb DNA fragment) As you can see, we can see the same result compared to other products.

A, 2x PCR Master mix Solution(i-TaqTM); B, Competitor Lane M, 1 Kb DNA Marker; lane NC, Negative control; lane 1, 25 ng DNA; lane 2, 12.5 ng DNA, lane 3, 6.25 ng DNA, lane 4, 3.125 ng DNA, lane 5, 1.56 ng DNA; lane 6, 0.753 ng DNA; lane 7, 0.38 ng DNA

TroubleShooting Guide
QI was using S taq, but recently I am looking for other products because of low sensitivity. We are trying to use it for generic PCR verification without large size. Is there an applicable product?
AWe recommend our i-Taq ™ DNA Polymerase. i-Taq ™ DNA Polymerase is the most basic taq for PCR and you can get satisfactory results in amplification of 1kb or less.
QThe size of the amplified gene is about 4kb. Is it ok to use i-Taq?
AThe recommended maximum amplification of our i-Taq ™ DNA Polymerase is 5kb. In addition, you will get the same efficiency as your i-MAXII ™ for amplification of products below 1kb.
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