Description

2x PCR Master mix type product with high specificity i-StarTaq™ DNA Polymerase with hot-start function to reduce nonspecific amplification

• • Ready to Use
     - All reagents required for the reaction are in solution in the tube   
     -  Immediate PCR by adding template and primer
• • PCR reaction volume control
• • High specificity and accuracy with hot-start function
• • High-purity Hot-start Taq DNA Polymerase
• • Highly reproducible results
• • Applicable to general PCR, RT-PCR, multiplex PCR and DNA discrimination by PCR (VNTR, STR, RAPD)
• • Applicable to DNA from cloned DNA to human genomic DNA 

2x PCR Master Mix Solution (i-StarTaq^TM) is a product designed to achieve the best results with simple, 2x master mix type in one tube, including i-i-StarTaq™ DNA Polymerase. This product is able to perform PCR by adding template DNA, primer set and D.W only, and gel loading buffer is included, so gel loading can be done without any other treatment. The i-StarTaq™ DNA Polymerase used in this product is a non-antibody-based, chemical-based hot-start enzyme that reduces nonspecific amplification and improves specificity and accuracy. It is a non-specific amplicon such as a nonspecific band or primer dimer It can be used as a solution when the singular band is weakly amplified. The 2x PCR Master Mix Solution (i-StarTaqTM), developed using this i-StarTaq™ DNA Polymerase, is capable of producing the results of high specificity and accuracy as well as the convenience mentioned above and it is highly reproducible.

Applications

• 01Pathogen detection
• 02Genomic DNA PCR
• 03Hot-start PCR
• 04Real-time PCR
• 05High GC rich, repeat region PCR
• 06T/A vector cloning
• 07LOH or MSI analysis related PCR, etc.

Kit Contents

Technical Data

In order to compare the sensitivity of 2x PCR Master Mix Solution (i-StarTaq^TM) with other products of the same function, 1 Kb DNA fragment PCR was performed by diluting human genomic DNA by concentration. Our kit shows better results.

A, 2x PCR Master mix Solution(i-StarTaq™); B, Competitor Lane M, 1 Kb DNA Marker; lane NC, Negative control; lane 1, 50 ng DNA; lane 2, 25 ng DNA, lane 3, 12.5 ng DNA, lane 4, 6.25 ng DNA, lane 5, 3.125 ng DNA; lane 6, 1.56 ng DNA; lane 7, 0.753 ng DNA

(780 bp), tsh (420 bp), and Irp2 (280 bp) to dilute the E. coli genomic DNA template in order to compare the sensitivity with 2x PCR Master Mix Solution (i-StarTaq^TM) As a result of multiplex PCR, we can confirm the superior results compared with other products.

A, 2x PCR Master mix Solution(i-StarTaq™); B, Competitor Lane M, 100 bp DNA Marker; lane NC, Negative control; lane 1, 100 ng gDNA, Lane 2, 50 ng gDNA; lane 3, 25 ng gDNA; lane 4, 12.5 ng gDNA; Lane 5, 6.25 ng gDNA; lane 6, 3.125 ng gDNA; lane 7, 1.5625 ng gDNA